ISSN:
1432-2242
Keywords:
Key words Pinus monticola D. Don
;
Chalcone synthase
;
Stilbene synthase
;
DNA probes
;
Conifers
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A probe for chalcone synthase (CHS) was generated by PCR using chalcone synthase conserved sequences. The cloned PCR product has high similarity to both chalcone synthase and stilbene synthase sequences. The probe was used to examine the organization of chalcone synthase and stilbene synthase genes in Abies procera, Pinus lambertiana, P. monticola, Picea glauca, P. sitchensis, Pseudostuga menziesii, Taxus brevifolia, and Thuja plicata. A large number of hybridizing bands were found in all species except T. plicata which did not cross hybridize. The hybridization patterns are highly polymorphic between the species and are also polymorphic within several of them.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001220050199
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