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  • 1
    Electronic Resource
    Electronic Resource
    (CAC)5 detects DNA fingerprints and sequences homologous to gene transcripts in rice (1994)
    Springer
    Biochemical genetics 32 (1994), S. 1-8 
    Details
    ISSN: 1573-4927
    Keywords: rice ; simple repetitive sequence ; DNA fingerprinting ; (CAC)5 ; poly(A)+ transcripts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In the present report the potential of (CAC)5 is demonstrated for DNA fingerprinting in rice. Based on the fingerprint data, (CAC)5 was estimated to differentiate up to 6.3×108 genotypes of rice. In a search for homologous sequences in rice transcripts, four oligonucleotide probes, namely, (CAC)5, (GATA)4, (GACA)4, and (TG)10, were used. Among these probes, (CAC)5 showed hybridization to total rice RNA in a tissue-specific manner; while a band at 1.2 kb was common in both seed and leaf RNA, there were additional prominent bands at 1.9 and 2.3 kb in seed and leaf RNA, respectively. When (CAC)5 was hybridized to poly(A)+ RNA, in addition to a band at 1.2 kb in seed and leaf, hybridization was observed only in seed to heterogeneously sized RNAs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00557234
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  • 2
    Electronic Resource
    Electronic Resource
    DNA fingerprinting to detect genetic variation in rice using hypervariable DNA sequences (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1000-1006 
    Details
    ISSN: 1432-2242
    Keywords: DNA fingerprinting ; Genetic variation ; Hypervariable DNA sequences ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The suitability of miniand microsatellite related DNA sequences capable of detecting multiple loci was investigated for their ability to generate DNA fingerprints in rice. These included R18.1, a cattle-derived probe, the M13 repeat probe, pV47, a human minisatellite probe; and repeats in the Per gene, telomere, chi sequence and 3′ hypervariable region of apolipoprotein B. With the R18.1, pV47 and M13 repeat probes, the level of polymorphism was high enough to identify all of the cultivars and wild rice species used in this study. R18.1, which showed the highest level of polymorphism, was estimated to identify up to 2.5×1020 genotypes of rice. In a F2 population of a ‘Basmati-370’ and ‘Taichung-65’ cross, loci detected by R18.1 segregated in a Mendelian fashion. DNA fingerprints were somatically stable and the hybridization patterns were identical among different plants of the same cultivar. Application of the above molecular genetic markers for identification of rice genotypes is reported here for the first time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222913
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  • 3
    Electronic Resource
    Electronic Resource
    DNA fingerprinting detects genetic variability in the pearl millet downy mildew pathogen (Sclerospora graminicola) (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 856-861 
    Details
    ISSN: 1432-2242
    Keywords: Downy mildew ; Microsatellites ; DNA fingerprinting ; Pearl millet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic variability in six host genotype-specific pathotypes of pearl millet downy mildew pathogen S. graminicola was studied at the molecular level using mini- and micro-satellites. Our results indicated that microsatellites (GAA)6, (GACA)4, and especially (GATA)4 were quite informative and showed high levels of polymorphism among the pathotypes. The six pathotypes could be classified into five groups based on the cluster analysis of their genetic similarities, thereby confirming the existence of distinct host genotype-specific virulence in S. graminicola pathotypes. We demonstrate, for the first time, the use of DNA fingerprinting to detect genetic variation in downy mildew fungus of pearl millet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223892
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  • 4
    Electronic Resource
    Electronic Resource
    Phylogenetic classification and molecular evolution of knotted1 homeobox genes (1999)
    Springer
    Theoretical and applied genetics 99 (1999), S. 203-209 
    Details
    ISSN: 1432-2242
    Keywords: Key words Homeobox gene ; KNOTTED1 ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Homeobox genes encode a family of DNA-binding regulatory proteins which are crucial for development. The first plant homeobox gene identified was knotted1 which plays a major role in leaf development. The knotted1 gene has a homeobox which encodes a homeodomain (HD) and HD proteins have been shown to function as transcription factors. A phylogenetic classification of the KNOTTED1 HD is presented. Here, we report six kn1 HDs from the cereals oat, barley, wheat, rye and rice. The KN1 class-I and -II genes can be divided into two distinct clades. Further, we hypothesize that KN1 and BELL1/MEIS HDs, (the closest non-KN1 class HDs) evolved from a common ancestor after divergence from the common precursor of all the homeobox genes. Our analysis clearly shows the presence of an ancestral KN1 HD from which all the known plant kn1 class of genes evolved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051226
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