ISSN:
1573-5044
Keywords:
DNA delivery
;
electroporation electrolyte
;
heat shock
;
Zea mays
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Electroporation was used for the delivery and subsequent expression of GUS and anthocyanin reporter genes into intact maize immature embryos. The optimal conditions consisted of culturing immature embryos for 4 days on N6 1-100-25-Ag medium prior to electroporation (375 V/cm; 960 µF capacitance) in EPR buffer containing DNA and 0.07 M sodium glutamate at room temperature (22°C) after a 10 min heat shock at 37°C. Under these conditions, over 40 spots of GUS transient activity were observed per immature embryo. Transient gene expression after electroporation was further demonstrated using an anthocyanin construct, which is specific for expression in plant cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01983234
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