Electronic Resource
Springer
Planta
149 (1980), S. 69-77
ISSN:
1432-2048
Keywords:
Chromatin
;
DNA
;
Hordeum Nucleus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A method for isolation and purification of intact nuclei from barley leaves was developed and several properties of the chromatin were studied. The dense structure of the main part of the chromatin does not alter the accessibility of the DNA to nucleases. 60% of the nuclear DNA can be degraded by micrococcal endonuclease. Nevertheless the solubility of the chromatin fragments depends on the extent of nuclease digestion; solubilisation occurring only when the major part of the internucleosomal DNA was degraded (≃30% of digestion). Electron microscopic observations suggest that this was due to particularly dense organization of the chromatin “in situ”. The possible physiological meaning of some of these properties are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00386230
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