Publication Date:
2000-06-02
Description:
Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae. Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G(1) phase ensures that initiation and elongation occur just once per cell cycle.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Labib, K -- Tercero, J A -- Diffley, J F -- New York, N.Y. -- Science. 2000 Jun 2;288(5471):1643-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Imperial Cancer Research Fund Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10834843" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Cycle Proteins/genetics/*metabolism
;
Chromosomal Proteins, Non-Histone
;
Chromosomes, Fungal/metabolism
;
*DNA Replication
;
DNA, Fungal/biosynthesis
;
*DNA-Binding Proteins
;
Fungal Proteins/genetics/*metabolism
;
G1 Phase
;
G2 Phase
;
*Ligases
;
Minichromosome Maintenance Complex Component 4
;
Minichromosome Maintenance Complex Component 6
;
Mitosis
;
Mutation
;
Recombinant Fusion Proteins/metabolism
;
Replicon
;
S Phase
;
Saccharomyces cerevisiae/genetics/*metabolism
;
*Saccharomyces cerevisiae Proteins
;
Temperature
;
*Ubiquitin-Protein Ligases
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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