Publication Date:
2012-11-01
Description:
To ensure their stable inheritance by daughter cells during cell division, bacterial low-copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between sister plasmids. In the ParMRC system of the Escherichia coli R1 plasmid, ParM, an actinlike protein, forms the spindle between ParRC complexes on sister plasmids. By using a combination of structural work and total internal reflection fluorescence microscopy, we show that ParRC bound and could accelerate growth at only one end of polar ParM filaments, mechanistically resembling eukaryotic formins. The architecture of ParM filaments enabled two ParRC-bound filaments to associate in an antiparallel orientation, forming a bipolar spindle. The spindle elongated as a bundle of at least two antiparallel filaments, thereby pushing two plasmid clusters toward the poles.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694215/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3694215/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gayathri, P -- Fujii, T -- Moller-Jensen, J -- van den Ent, F -- Namba, K -- Lowe, J -- MC_U105184326/Medical Research Council/United Kingdom -- U.1051.04.014(78932)/Medical Research Council/United Kingdom -- U105184326/Medical Research Council/United Kingdom -- New York, N.Y. -- Science. 2012 Dec 7;338(6112):1334-7. doi: 10.1126/science.1229091. Epub 2012 Oct 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23112295" target="_blank"〉PubMed〈/a〉
Keywords:
Actin Cytoskeleton/*chemistry/*metabolism
;
Actins/*chemistry/*metabolism
;
Adenylyl Imidodiphosphate/chemistry/metabolism
;
Cell Division/*genetics
;
Cryoelectron Microscopy
;
DNA, Bacterial/chemistry/*metabolism
;
Escherichia coli/*genetics/physiology
;
Escherichia coli Proteins/*chemistry/*metabolism
;
Protein Conformation
;
R Factors/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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