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  • DGJ0: Medium of De Greef and Jacobs (1979) lacking BA  (1)
  • Gluconeogenesis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of in vitro sugar beet (Beta vulgaris L.) shoot tips by a vitrification technique (2000)
    Springer
    Plant cell reports 19 (2000), S. 1064-1068 
    Details
    ISSN: 1432-203X
    Keywords: Key words Beta vulgaris L. Cryopreservation ; Shoot tips ; Encapsulation-dehydration ; Vitrification ; AbbreviationsBA: N-6-benzylaminopurine ; CMS:¶Cytoplasmic male sterile ; DMSO: Dimethylsulfoxide ; DGJ: Medium of De Greef and Jacobs (1979) ; DGJ0: Medium of De Greef and Jacobs (1979) lacking BA ; IBA: Indole-3-butyric acid ; kin: 6-Furfurylaminopurine ; LS: Loading solution ; PAR: Photosynthetic active radiation ; PVS2: Plant vitrification solution ; RS: Recovery solution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sugar beet shoot tips from cold-acclimated plants were successfully cryopreserved using a vitrification technique. Dissected shoot tips were precultured for 1 day at 5  °C on solidified DGJ0 medium with 0.3 M sucrose. After loading for 20 min with a mixture of 2 M glycerol and 0.4 M sucrose (20  °C), shoot tips were dehydrated with PVS2 (0  °C) for 20 min prior to immersion in liquid nitrogen. Both cold acclimation and loading enhanced the dehydration tolerance of shoot tips to PVS2. After thawing, shoot tips were deloaded for 15 min in liquid DGJ0 medium with 1.2 M sucrose (20  °C). The optimal exposure time to both loading solution and PVS2 depended on the in vitro morphology of the clone. With tetraploid clones a higher sucrose concentration during cold acclimation and preculture further enhanced survival after cryopreservation. Survival rates ranged between 60% and 100% depending on the clone. Since only 10–50% of the surviving shoot tips developed into non-hyperhydric shoots, regrowth was optimized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000232
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  • 2
    Electronic Resource
    Electronic Resource
    Identification and characterization of regulatory elements in the phosphoenolpyruvate carboxykinase gene PCK1 of Saccharomyces cerevisiae (1995)
    Springer
    Molecular genetics and genomics 246 (1995), S. 367-373 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Phosphoenolpyruvate carboxykinase ; Glucose repression ; Gluconeogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphoenolpyruvate carboxykinase is a key enzyme in gluconeogenesis. The expression of the PCK1 gene in Saccharomyces cerevisiae is strictly regulated and dependent on the carbon source provided. Two upstream activation sites (UAS1PCK1 and UAS2PCK1) and one upstream repression site (URSPCK1) were localized by detailed deletion analysis. The efficacy of these three promoter elements when separated from each other was confirmed by investigations using heterologous promoter test plasmids. Activation mediated by UAS1PCK1 or UAS2PCK1 did not occur in the presence of glucose, indicating that these elements are essential for glucose derepression. The repressing effect caused by URSPCK1 was much stronger in glucose-grown cells than in ethanol-grown cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288610
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