ISSN:
1573-4935
Keywords:
D-glucose
;
D-glyceraldehyde
;
pH (cytoplasmic)
;
pancreatic B cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The effects of D-glucose, D-glyceraldehyde, glibenclamide, D-600, NH 4 + and high concentrations of K+ on cytoplasmic pH (pH i ) were investigated in dispersed and cultured pancreatic β-cells fromob/ob mice. The cytoplasmic pH was measured with the fluorescent H+-indicator quene 1. The average pH i value in resting β-cells was 6.71. Addition of 20 mM of the physiological stimulus D-glucose increased pH i with 0.05 units. Both glibenclamide and high concentrations of K+ decreased pH i . The latter effects were completely reversed by D-600, supporting the notion that free cytoplasmic Ca2+ can be involved in the regulation of pH i . In contrast to D-glucose, 10mM of D-glyceraldehyde decreased pH i by 0.09 units, an effect persisting even in the presence of D-600. From the present study it is evident that D-glyceraldehyde and D-glucose have opposite effects on pH i in pancreatic β-cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01116422
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