ISSN:
1573-0778
Schlagwort(e):
Cytotoxicity
;
mercury
;
primary renal culture
;
proximal tubular cells
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
Abstract Mercuric compound toxicity is well documented in animals and man for practically all organs. The recent development of cell culture techniques appeared as a novel fruitful tool in toxicology, especially in renal toxicology. Heavy metal induced renal cell alterations can be evaluated by membrane permeability damages. The present study evaluates mercuric chloride nephrotoxic effect in human kidney epithelial cells by measuring the release of two specific nephrotoxicity marker enzymes, Gamma Glutamyl Transferase (GGT) and Alkaline Phosphatase (ALP) in the culture medium. Cultured kidney epithelial cells were exposed to different HgCl2 concentrations (5, 10, 20, and 50 μg). Cultures were examined after 6 and 24 hours exposure. A good correlation between mercury dose and toxic effect, and exposure time and toxic effect was found. Enzymes were significantly released into the culture medium for 5 μg and 10 μg HgCl2/ml after 6 hours exposure; and after 24 hours exposure, enzymes were released for 5 μg/ml only. It appears that the specific tubular enzyme release in the culture medium is a good in vitro test for quantification of specific tubular damage.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00145030
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