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Digitale Medien

The involvement of F-actin inUromyces cell differentiation: The effects of cytochalasin E and phalloidin (1986)

Tucker, B. E. ; Hoch, H. C. ; Staples, R. C.

Springer

Protoplasma 135 (1986), S. 88-101

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ISSN: 1615-6102

Schlagwort(e): Uromyces ; Differentiation ; Cytoskeleton ; Actin ; Cytochalasin E ; Phalloidin

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The role of F-actin in cell differentiation ofUromyces appendiculatus (bean rust fungus) germlings was examined by treating differentiating and nondifferentiating germlings with the actin-binding drugs cytochalasin E (CE) and phalloidin. Prolonged exposure of urediospores to 5×10−3–5 × 10−5 M CE induced nuclear division in up to 28–45% of the resulting germlings, whereas the rate of mitosis in established germlings exposed to these concentrations of CE was significantly lower (4–11%). Germlings treated with CE shifted from polarized apical growth to spherical expansion, cytoplasmic microfilaments were depolymerized, and nuclear inclusions became enlarged. Differentiating germlings exposed to a 10 minute pulse of 5×10−6M CE before the initiation of septum formation prevented the establishment of the F-actin septal ring and growth of the crosswall delimiting the appressorium. Although these CE treatments resulted in morphological and nuclear events similar to those occurring during normal appressorium formation, transient microfilament depolymerization was not sufficient to induce differentiation. Phalloidin stabilized cytoplasmic microfilaments, especially posteriorly-located microfilaments, but did not affect differentiation, nor did it significantly inhibit the effects of CE.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01277002

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Digitale Medien

The microtubule cytoskeleton in hyphae ofUromyces phaseoli germlings: Its relationship to the region of nucleation and to the F-actin cytoskeleton (1985)

Hoch, H. C. ; Staples, R. C.

Springer

Protoplasma 124 (1985), S. 112-122

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ISSN: 1615-6102

Schlagwort(e): Cytoskeleton ; Immunocytochemistry ; Microfilaments ; Microtubules ; Microtubule organizing center ; Uromyces phaseoli

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The microtubule and F-actin cytoskeleton of nondifferentiated germlings ofUromyces phaseoli was studied using immunofluorescence methodologies. The microtubules were oriented mostly parallel to the longitudinal axis of the hypha. Microtubule depolymerizing agents, such as cold, demecolcine, griseofulvin and nocodazole, were effective in destroying the microtubule network, but not the F-actin system. Repolymerization of microtubules, following release from these agents, occurred first in the hyphal apices and not near the nuclei or spindle pole bodies. It was concluded that the microtubule nucleating region in such fungal cells is located in the apical regions. Enhanced microtubule arrays were visualized following incubation of the cells in taxol, an agent known to favor microtubule polymerization.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01279730

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