Publication Date:
2009-03-28
Description:
To generate efficient vaccines and cures for Mycobacterium tuberculosis, we need a far better understanding of its modes of infection, persistence, and spreading. Host cell entry and the establishment of a replication niche are well understood, but little is known about how tubercular mycobacteria exit host cells and disseminate the infection. Using the social amoeba Dictyostelium as a genetically tractable host for pathogenic mycobacteria, we discovered that M. tuberculosis and M. marinum, but not M. avium, are ejected from the cell through an actin-based structure, the ejectosome. This conserved nonlytic spreading mechanism requires a cytoskeleton regulator from the host and an intact mycobacterial ESX-1 secretion system. This insight offers new directions for research into the spreading of tubercular mycobacteria infections in mammalian cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770343/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2770343/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hagedorn, Monica -- Rohde, Kyle H -- Russell, David G -- Soldati, Thierry -- AI 067027/AI/NIAID NIH HHS/ -- HL 055936/HL/NHLBI NIH HHS/ -- R01 AI067027/AI/NIAID NIH HHS/ -- R01 AI067027-04/AI/NIAID NIH HHS/ -- R01 HL055936/HL/NHLBI NIH HHS/ -- R01 HL055936-09/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2009 Mar 27;323(5922):1729-33. doi: 10.1126/science.1169381.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departement de Biochimie, Faculte des Sciences, Universite de Geneve, Sciences II, 30 quai Ernest Ansermet, CH-1211 Geneve-4, Switzerland.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19325115" target="_blank"〉PubMed〈/a〉
Keywords:
Actins/*physiology
;
Animals
;
Bacterial Proteins/metabolism
;
Cell Membrane/microbiology
;
Cytoskeleton/*microbiology/physiology/ultrastructure
;
Cytosol/microbiology
;
Dictyostelium/*microbiology/ultrastructure
;
GTP Phosphohydrolases/metabolism
;
Mycobacterium avium/genetics/pathogenicity/*physiology
;
Mycobacterium marinum/genetics/pathogenicity/*physiology
;
Mycobacterium tuberculosis/genetics/pathogenicity/*physiology
;
Phagocytosis
;
Pressure
;
Vacuoles/microbiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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