ISSN:
1432-072X
Keywords:
Transmembrane proton gradient
;
Desulfovibrio desulfuricans CSN
;
31P NMR
;
Cytoplasmic pH
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The transmembrane proton gradient of the sulfate-reducing bacterium Desulfovibrio desulfuricans strain CSN has been determined by in vivo31P nuclear magnetic resonance (NMR) spectroscopy in the absence of dioxygen. At pH 7.0 in the medium (pHex) the intracellular pH (pHin) was 7.5. By lowering pHex to 5.9 pHin decreased to 7.1. At pHex greater than 7.7 the transmembrane proton gradient (ΔpH) was zero. The uncouplers 3,3′,4′,5-tetrachlorosalicylanilide (TCS) and carbonylcyanide-m-chlorophenylhydrazone (CCCP), or the permeant anion thiocyanate caused complete dissipation of ΔpH.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290988
Permalink