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  • 1
    Electronic Resource
    Electronic Resource
    A comparison between mitochondrial DNA of an isogenic male-sterile (5) and male-fertile (F) couple (HA89) of sunflower (1985)
    Springer
    Current genetics 9 (1985), S. 245-251 
    Details
    ISSN: 1432-0983
    Keywords: Cytoplasmic male sterility ; Hehanthus annuus ; mt-DNA ; Sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ultracentrifugation. 2. In polyacrylamide gel it is found that mitochondria from F cytoplasm contain a Low-Molecular-Weight (LMW) mt-DNA molecule of 1.45 Kilobase-pairs (kb) in addition to the major mt-DNA. On the contrary, mitochondria from S cytoplasm contain no LMW molecule. Treatments with DNAse, RNAse and Nuclease S1 show that this LMW mt-DNA molecule consists of a supercoiled circular DNA. The length and form of the LMW molecule are confirmed by electron microscopy observations. 3. The mt-DNAs from S and F cytoplasms have also been investigated using restriction endonuclease digestions (Sa11, Xho1, Bg11). The electrophoresis of the resulting fragments reveals several differences between the S and F cytoplasm. It is not known whether the differences observed between F and S mt-DNA are correlated with the cytoplasmic male sterility phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00420319
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  • 2
    Electronic Resource
    Electronic Resource
    Histones and nucleosomes in Archaea and Eukarya: a comparative analysis (1998)
    Springer
    Extremophiles 2 (1998), S. 141-148 
    Details
    ISSN: 1433-4909
    Keywords: Key words Hyperthermophile ; Methanothermus fervidus ; Histone evolution ; Genome structure ; Nucleosome positioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Archaeal histones from mesophilic, thermophilic, and hyperthermophilic members of the Euryarchaeota have primary sequences, the histone fold, tertiary structures, and dimer formation in common with the eukaryal nucleosome core histones H2A, H2B, H3, and H4. Archaeal histones form nucleoprotein complexes in vitro and in vivo, designated archaeal nucleosomes, that contain histone tetramers and protect approximately 60 base pairs of DNA from nuclease digestion. Based on the sequence and structural homologies and experimental data reviewed here, archaeal nucleosomes appear similar, and may be homologous in evolutionary terms and function, to the structure at the center of the eukaryal nucleosome formed by the histone (H3+H4)2 tetramer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050053
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