Publication Date:
2004-11-20
Description:
The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ando, Ryoko -- Mizuno, Hideaki -- Miyawaki, Atsushi -- New York, N.Y. -- Science. 2004 Nov 19;306(5700):1370-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama, 351-0198, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15550670" target="_blank"〉PubMed〈/a〉
Keywords:
Active Transport, Cell Nucleus
;
Amino Acid Sequence
;
Animals
;
Anthozoa
;
COS Cells
;
Cell Nucleus/*metabolism
;
Cytoplasm/*metabolism
;
Epidermal Growth Factor/pharmacology
;
Fluorescence
;
HeLa Cells
;
Humans
;
Hydrogen-Ion Concentration
;
Light
;
Luminescent Proteins/chemistry/*metabolism
;
MAP Kinase Signaling System
;
Microscopy, Confocal
;
Mitogen-Activated Protein Kinase 3/*metabolism
;
Molecular Sequence Data
;
Nuclear Envelope/*metabolism
;
Phosphorylation
;
Protein Transport
;
Recombinant Proteins/chemistry/metabolism
;
Transfection
;
beta Karyopherins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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