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  • 1
    Electronic Resource
    Electronic Resource
    The effect of deuterium oxide on protein turnover in Lemna minor (1979)
    Springer
    Planta 146 (1979), S. 229-236 
    Details
    ISSN: 1432-2048
    Keywords: Density labelling ; Deuterium oxide ; Lemna ; Protein turnover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lemna minor fronds transferred to a sterile culture medium containing 50% (v/v) deuterium oxide (2H2O) rapidly undergo a loss of soluble protein with a corresponding increase in free amino acids. The loss of protein is due to two factors: (i) the inhibition of protein synthesis for 4 h followed by a slower rate of synthesis than normal, (ii) a rapid 9–10 fold increase in protein degradation. In plants grown for longer periods (3–6 days) in 50% 2H2O medium, protein synthesis is inhibited by 20% and the rate constant of degradation is 2–3 times that measured in fronds growing in normal (H2O containing) complete medium. The initial loss of protein is not due to the breakdown of any specific protein fraction. Investigation of several enzymes indicates that all proteins are catabolised in response to 2H2O treatment. The implications of these results with regard to the interpretation of density-labelling experiments are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388237
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  • 2
    Electronic Resource
    Electronic Resource
    Hormonal control of tobacco protoplast nucleic acid metabolism during in vitro culture (1981)
    Springer
    Planta 152 (1981), S. 1-7 
    Details
    ISSN: 1432-2048
    Keywords: Auxin ; Cytokinin ; DNA ; Nicotiana RNA ; Protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tobacco mesophyll protoplasts cultivated in vitro do not synthesize a measurable quantity of chloroplastic ribosomal RNA, but actively synthesize cytoplasmic ribosomal RNA, polyadenylated RNA, and proteins. These syntheses are essentially independent of the presence of hormones in the culture medium and are thus related to the ageing phenomenon induced by isolation from the plant and in-vitro culture. At all stages of culture and in all culture media, protoplasts incorporate low levels of thymidine into their DNA. However, the incorporation of considerable quantities of thymidine, indicative of the S phase, only takes place after 25–30 h and requires the presence of auxin and cytokinin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384976
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    Paper (German National Licenses)
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