Publication Date:
2000-10-06
Description:
Posttranscriptional gene silencing (PTGS) is an ancient eukaryotic regulatory mechanism in which a particular RNA sequence is targeted and destroyed. The helper component-proteinase (HC-Pro) of plant potyviruses suppresses PTGS in plants. Using a yeast two-hybrid system, we identified a calmodulin-related protein (termed rgs-CaM) that interacts with HC-Pro. Here we report that rgs-CaM, like HC-Pro itself, suppresses gene silencing. Our work is the first report identifying a cellular suppressor of PTGS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Anandalakshmi, R -- Marathe, R -- Ge, X -- Herr, J M Jr -- Mau, C -- Mallory, A -- Pruss, G -- Bowman, L -- Vance, V B -- New York, N.Y. -- Science. 2000 Oct 6;290(5489):142-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, University of South Carolina, Columbia, SC 29208, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11021800" target="_blank"〉PubMed〈/a〉
Keywords:
Agrobacterium tumefaciens/genetics
;
Amino Acid Sequence
;
Cysteine Endopeptidases/*metabolism
;
*Gene Silencing
;
Genes, Plant
;
Green Fluorescent Proteins
;
Luminescent Proteins/genetics
;
Molecular Sequence Data
;
Plant Proteins/chemistry/genetics/*metabolism
;
Plant Tumors/genetics
;
Plants, Genetically Modified
;
*Plants, Toxic
;
Plasmids
;
Potexvirus/genetics
;
RNA, Messenger/genetics/metabolism
;
RNA, Plant/genetics/metabolism
;
Tobacco/*genetics/metabolism
;
Transcription, Genetic
;
Transgenes
;
Viral Proteins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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