ISSN:
1617-4623
Keywords:
Human HPRT gene
;
Autonomously replicating sequence
;
Extrachromosomal DNA
;
Matrix association region
;
Bent DNA
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A sequence that supports extrachromosomal replication of plasmids in yeast has been identified within the first intron of the human hypoxanthine-guanine phosphori bosyltransferase (HPRT) gene. This represents the first isolation of such an autonomously replicating sequence (ARS) from an exactly known position in the human genome. This ARS shares similarities of imparted yeast phenotype and DNA sequence with other heterologus ARSs. In addition, this sequence is found to be a matrix association region (MAR) on the basis of specific binding to nuclear matrices prepared from several mammalian cell types. It also exhibits anomalous electrophoretic behavior, characteristic of bent DNA, on polyacrylamide gels. The coincidence of these properties supports the possibility that this region may play a role in DNA replication within its normal chromosomal context.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00334700
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