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  • 1
    Electronic Resource
    Electronic Resource
    Estimation of the genome size of blue-green algae from DNA renaturation rates (1974)
    Springer
    Archives of microbiology 99 (1974), S. 251-254 
    Details
    ISSN: 1432-072X
    Keywords: Blue-Green Algae ; DNA Renaturation ; Genome Size
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The molecular weight of the genomes of the blue-green algaeAnacystis nidulans andAnabaena cylindrica have been estimated as 2.27×109 and 2.47×109 daltons respectively from the renaturation kinetics of DNA. Thus the genomes of these organisms are similar in size to that ofEscherichia coli K-12, (2.40×109 daltons) measured by the same technique. No evidence was obtained of repeated sequences in the DNA of the two blue-green algae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00696239
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  • 2
    Electronic Resource
    Electronic Resource
    Mutation of the cyanobacterium Anacystis nidulans (Synechococcus PCC 6301): Improved conditions for the isolation of auxotrophs (1980)
    Springer
    Archives of microbiology 124 (1980), S. 177-184 
    Details
    ISSN: 1432-072X
    Keywords: Anacystis nidulans ; Auxotroph ; Cyanobacteria ; Mutant isolation ; Synechococcus PCC 6301 ; Ethyl methanesulphonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous attempts to isolate auxotrophic mutants of Anacystis nidulans produced only a limited range of phenotypes. The frequency of recovery of auxotrophic mutants has been quantified following different mutagenic and selective treatments, and their yield has been improved by using (1) a complete medium, (2) additional mutagens, (3) multiple cycles of penicillin enrichment and (4) altered pre-enrichment starvation conditions. These modified induction and selection conditions permitted the isolation of mutants defective in nitrate reductase, nitrite reductase or malate dehydrogenase, unable to reduce sulphate, or deficient in the synthesis of biotin, thiamine, paminobenzoate, serine, glutamate, adenine or uracil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427724
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  • 3
    Electronic Resource
    Electronic Resource
    Effect of iron and other nutrient limitations on the pattern of outer membrane proteins in the cyanobacterium Synechococcus PCC7942 (1989)
    Springer
    Archives of microbiology 152 (1989), S. 224-228 
    Details
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Synechococcus ; Cell wall ; Outer membrane polypeptides ; Iron ; Magnesium ; Phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When cells of Synechococcus PCC7942 were subjected to either iron or magnesium limitation, there was an appearance of specific proteins in the outer membrane (isolated as the cell wall fraction). Under iron limitation outer membrane polypeptides of M r 92000, 48000–50000 and 35000 appeared. Specific iron-limited outer membrane proteins (IRMPs) of M r 52000 and 36000 were also induced in iron-limited cultures of Synechocystis PCC6308. Under magnesium limitation polypeptides of M r 80000, 67000, 62000, 50000, 28000 and 25000 appeared in the outer membrane. phosphate limitation caused minor changes in the outer membrane protein pattern, with polypeptides of M r 32000 and one of over 100000 being induced, whereas calcium limitation had no apparent affect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409655
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