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  • 1
    Electronic Resource
    Electronic Resource
    Electron microscopy and X-ray microanalysis of a halophilic leptospire (1981)
    Springer
    Archives of microbiology 130 (1981), S. 339-343 
    Details
    ISSN: 1432-072X
    Keywords: Leptospira ; Halophilic ; Electron microscopy ; X-ray analysis ; Inclusions ; Cytoplasmic tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology of cells of strain Muggia, a slightly halophilic leptospire, was examined by the negative staining technique. The ultrastructure of the cells was rather similar to that of cells of Leptonema illini, i. e. the cells possessed cytoplasmic tubules. The basal complex of their flagella, however, was similar to the corresponding part of flagella on Gramnegative bacteria. The interior of the cells was densely packed with inclusions, except for the two outermost wavelengths at each end where these inclusions were absent. X-ray microanalysis showed that the inclusions contained sodium and chlorine as their main constituents. The inclusions disappeared upon storage of the cultures at room temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414596
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  • 2
    Electronic Resource
    Electronic Resource
    Low temperature techniques for X-ray microanalysis in pathology: Alternatives to cryoultramicrotomy (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 9 (1988), S. 83-98 
    Details
    ISSN: 0741-0581
    Keywords: X-ray microanalysis ; Pathology ; Diagnosis ; Cryo(ultra)-microtomy ; Cultured cells ; Freeze-drying ; Freeze-substitution ; Low-temperature embedding ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Many diseases are associated with a change in the distribution of diffusible ions at the cell or tissue level. These diseases can profitably be studied by X-ray microanalysis. This technique for the study of ion distribution requires the use of cryoprepared specimens. Analysis at low or medium resolution can be carried out on thick or semi-thick cryosections, or on frozenhydrated or freeze-dried embedded bulk samples. Such analyses are particularly useful in the initial stages of an investigation or when data from a large number of samples have to be acquired. Also X-ray microanalysis of cultured or single cells prepared by freeze-drying can be used to rapidly collect information on a large number of cells. Analysis at high resolution has to be carried out on thin sections: Cryosections or sections of freeze-substituted or freeze-dried embedded tissue. For the latter type of specimens, the use of low-temperature embedding methods may have important advantages.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060090108
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