ISSN:
1615-6102
Keywords:
Uromyces appendiculatus
;
High pressure freezing
;
Freeze substitution
;
Cryoprotection
;
Fine structure preservation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The fine structure of the intercellular dikaryotic hyphae of the biotrophic fungusUromyces appendiculatus was studied. High pressure freezing and freeze substitution were used to achieve a closer approximation of the native state than with conventional fixation and dehydration techniques. In addition to organelles previously described in rust fungi, heavily decorated multivesicular bodies (star bodies) were found close to the nuclei. Two types of tubular-vesicular complexes were distributed randomly within the cytoplasm of the hyphae. Furthermore, a more or less pronounced brush-like fibrillar layer on the hyphal walls was detected. The possibility that the latter two structures are correlated with the biotrophic phase of this fungus is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01403336
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