ISSN:
1615-6102
Keywords:
Cryopreservation
;
High pressure freezing
;
Actinomycete
;
Nitrogen-fixation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Actinorhizal root nodules ofGymnostoma papuanuum (Casuarinaceae) were examined with transmission electron microscopy after being either fixed with glutalaldehyde and osmium tetroxide or frozen with liquid nitrogen at high pressure and freeze-substituted. Much better preservation was obtained by the cryopreservation method. Mitochondria, plastids, membranes und ribosomes were much better preserved in the frozen specimens than in the chemically fixed tissues. No nucleoids were observed in the microsymbiont in frozen specimens. In contrast nucleoid regions were present in chemically fixed specimens. The actinomycete microsymbiont differentiates small spherical-shaped symbiotic vesicles after the hyphae have grown and penetrated into most regions of the cytoplasm.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01322650
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