ISSN:
1432-072X
Keywords:
Beggiatoa alba
;
Glutamine synthetase
;
Glutamate synthase
;
Alanine dehydrogenase
;
Methionine sulfoximine
;
Ammonia metabolism
;
Covalent modification
;
Nitrogen metabolism
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Beggiatoa alba B18LD was investigated for its pathways of ammonia assimilation. The increase in growth yields ofB. alba with excess acetate was linear from 0.1 to 2.0 mM ammonia.B. alba had strong glutamine synthetase (GS) and glutamate synthase (GOGAT) activities, irrespective of the ammonia concentration in the medium. Glutamate dehydrogenase activity was not found, and alanine dehydrogenase (aminating) was observed only whenB. alba was grown at high (2.0 mM) ammonia. Methionine sulfoximine, an inhibitor of GS, inhibited growth ofB. alba irrespective of the ammonia concentration in the medium. Thus it appears the primary pathway for ammonia assimilation inB. alba is via the GS-GOGAT pathway at both low and high ammonia concentrations. Preliminary experiments were unable to discern if theB. alba GS is modified by covalent modification.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00693403
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