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  • Concanavalin A  (1)
  • kairomone  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 72 (1994), S. 1-10 
    ISSN: 1570-7458
    Keywords: lectins, peritrophic membrane ; Lucilia cuprina ; blowfly strike ; larvicide ; feeding deterrent ; wheat germ lectin ; lentil lectin ; Concanavalin A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Larvae of the blowflyLucilia cuprina (Wied.) (Diptera: Calliphoridae) were grownin vitro on a serum-free medium in the presence of a number of lectins. Lectins with specificities for β-(1,4)-N-acetylglucosamine (wheat germ lectin) and α-D-mannopyranosyl and (α-D-glucopyranosyl residues (lentil lectin and Con A) caused strong concentration-dependent inhibition of the growth of the larvae and substantial mortality. Wheat germ lectin had the strongest effects, showing 50% inhibition of larval growth at a concentration of 2 μM and 100% mortality at 25 μM. Other lectins with different sugar specificities had much less effect. The mechanism of the larvicidal action(s) of wheat germ lectin, lentil lectin and Con A was investigated. There were at least three effects of these lectins onL. cuprina larvae. First, these lectins bound to and reduced the permeability of the peritrophic membrane of the larvae. Second, they reduced ingestion of diet medium by larvae. Third, the lectins also bound to the apical membranes of larval gut epithelial cells although there were no obvious signs of damage to these cells. It is concluded that the combination of these effects probably results in the starvation of the larvae. The implications of these results in terms of possible control strategies forL. cuprina are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 62 (1992), S. 125-130 
    ISSN: 1570-7458
    Keywords: Dacus tryoni ; Tephritidae ; fruit fly ; olfactometer ; oviposition ; behaviour ; fruit ; kairomone ; attractant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The vapours of certain pure chemicals, typical of ripe fruits, elicited characteristic components of ovipositional behaviour from gravid Dacus tryoni (Froggat) in an olfactometer: the flies walked and flew upwind to the source of the vapour and then probed with their ovipositors. A range of alcohols, acids, ketones and esters having 2–6 carbon atoms were effective (1 and 10% of iso-butyric acid, n-butyric acid, methyl butyrate, ethyl butyrate, 2-butanone, ethyl lactate and ethyl acetate; and 10% concentrations of ethanol and 2-propanone). The most effective were 4–6 carbon acids, esters and ketones. Behavioural threshold for n-butyric acid vapour at 26°C was obtained from a 5×10−3% dilution in paraffin oil; maximum fly response occurred at about 200 times this concentration. Low concentrations of the 15-carbon sesquiterpene, α-farnesene, were also very effective, despite its lower volatility. These results suggest that at least three different types of alfactory sensory neurones are involved in the identification of fruit attractants by gravid D. tryoni.
    Type of Medium: Electronic Resource
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