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Mobilization of cloned luciferase genes into Vibrio harveyi luminescence mutants (1986)

Gupta, Subhash C. ; Reese, Catherine Potrikus ; Hastings, J. Woodland

Springer

Archives of microbiology 143 (1986), S. 325-329

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ISSN: 1432-072X

Keywords: Plasmid mobilization ; Bioluminescence ; Temperature conditional ; Complementation ; Marine bacteria ; Autoinduction ; Vibrio harveyi ; Luciferase genes ; Cloning

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A recombinant plasmid which carried a 5 kb fragment of Vibrio harveyi DNA containing the luxA and luxB genes was mobilized from Escherichia coli into luminescence-deficient mutants of V. harveyi. The cloned genes complemented a temperature sensitive luciferase mutation, but failed to complement lesions in two different aldehyde deficient mutants. Expression of the cloned genes was not subject to autoinduction in either E. coli or in V. harveyi.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00412797

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