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Complementation of anArabidopsis thaliana biotin auxotroph with anEscherichia coli biotin biosynthetic gene (1996)

Patton, D. A. ; Volrath, S. ; Ward, E. R.

Springer

Molecular genetics and genomics 251 (1996), S. 261-266

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ISSN: 1617-4623

Keywords: Biotin ; Arabidopsis ; Complementation ; bioA

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract TheArabidopsis thaliana biotin auxotrophbio1 was rendered prototrophic by transformation with a chimeric transgene containing theEscherichia coli bioA gene driven by a constitutive promoter. ThebioA gene encodes the biotin biosynthetic enzyme 7,8-diaminopelargonic acid aminotransferase. Unlike the untransformed control plants, transgenic plants expressing the bacterial transgene synthesized biotin and grew to maturity without biotin-deficiency symptoms. These findings demonstrate thatbio1/bio1 mutant plants are defective in the gene encoding 7,8-diaminopelargonic acid aminotransferase.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02172516

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