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  • 1
    Electronic Resource
    Electronic Resource
    An enhanced liquid chromatographic method for 5-hydroxymethylfurfural determination in UHT milk (1992)
    Springer
    Chromatographia 33 (1992), S. 45-48 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Heat-treated milk ; Milk quality indicators ; 5-Hydroxymethyl-2-furfuraldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An enhanced method of HPLC for the determination of 5-Hydroxymethyl-2-furfuraldehyde (HMF) in fluid milk was perfected. Its resolving capacity permitted the specific separation of this compound. The real concentration (μmol/l) of HMF was determined and not the HMF value, as in the colorimetric analysis, where an intermediary coloured compound (HMF-TBA) is utilised. The optimum pH condition (pH=4.0–4.2) for the mobile phase to prevent overlapping between the HMF peak and the peaks of the other compound (α and β) that coeluted with the HMF and also the optimum organic solvent content (3% or 7%) were established. This is a sensitive, quick and safe method for the determination of HMF in fluid milk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02276850
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  • 2
    Electronic Resource
    Electronic Resource
    An improved method for determination of the activity of β-galactosidase in yoghurt by high-performance liquid chromatography (1996)
    Springer
    Chromatographia 43 (1996), S. 85-88 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Yoghurt ; β-Galactosidase activity ; Glucose ; Galactose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new method has been developed for the HPLC determination of the activity of lactase from the microorganisms in yoghurt. The method is based upon the ability of β-galactosidase to hydrolyze lactose bonds in glucose and galactose. To determine such activity in yoghurt, 1 mL of yoghurt was diluted 1∶10 with 0.1 M phosphate buffer (pH 7) containing 2 % (w/v) lactose and 5 mM dithioerythritol as reducing agent; the mixture was the incubated and injected into the HPLC. Lactase activity remained high as long as the number of viable microorganisms did not fall below the minimum CFU·mL−1 (107). This method is more repeatable than conventional colorimetric determination, and may also be automated for routine analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02272828
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    Paper (German National Licenses)
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