ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Micro enzyme-immobilized columns
;
Separation of aldehydes
;
Derivatization and fluorometric detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Aldehydes in liquors were analyzed by micro high-performance liquid chromatography with post-column derivatization on the enzyme-immobilized glass beads. β-nicotinamide adenine dinucleotide (NAD) was converted to its reduced form (NADH) on the enzyme-immobilized column in the presence of an aldehyde and a thiol, followed by fluorometric detection. A detection limit of 2.5 ppm (50 pg) was achieved for acetaldehyde.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02324822
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