ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Fluorimetric detection
;
Adriamycin
;
Adriamycinol
;
Human plasma
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A simple and very sensitive HPLC method, for the simultaneous determination in human plasma of adriamycin and its metabolite adriamycinol, is described. Plasmas from patients were stored frozen. Thawed samples were extracted by absorption of anthracyclin onto a small C18 column. After evaporation of the eluate and reconstitution of the residue with methanol (100μL), 30 to 40μL of the mixture were injected into the chromatograph. Separation was obtained using an RP 8 column with a mobile phase of formate buffermethanol-acetonitrile (50∶23∶27, v/v). A spectrofluorimeter was used as detector. The limit of sensitivity of the assay was 50 pcg/ml of plasma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02261018
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