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  • 1
    Electronic Resource
    Electronic Resource
    Ion-pair reversed phase HPLC determination of nucleotides, nucleosides and nucleobases — Application to nucleotide metabolism in hepatocytes (1989)
    Springer
    Chromatographia 27 (1989), S. 639-643 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase ; Ion-pair ; Recoveries ; Hepatocytes ; Purine compounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Three groups of metabolites were analyzed in extracts of rat hepatocytes by an HPLC method: (i) nucleotides (ATP, ADP, AMP, GTP, GDP, UTP, UDP, IMP, UMP), (ii) nucleosides and nucleobases (adenosine, adenine, guanosine, inosine, xanthine, hypoxanthine, uridine) and (iii) inhibitors of xanthine oxidoreductase (oxypurinol, allopurinol). Perchloric acid extracts were neutralized with K2CO3/triethanolamine and analyzed at 254 nm by reversed-phase ion-pair high-performance liquid chromatography. The nucleotides and their derivatives were separated with a gradient elution using 10 mM NH4H2PO4/2 mM t-butylammonium-phosphate and acetonitrile. Recovery values were estimated for the extraction procedure used. The method was applied to the investigation of nucleotide metabolism of hepatocytes of starved rats at anoxia and reoxygenation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02258995
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  • 2
    Electronic Resource
    Electronic Resource
    Nucleotide and aldehyde analysis by HPLC for determination of radical induced damage (1989)
    Springer
    Chromatographia 28 (1989), S. 65-68 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Oxygen radicals ; Nucleotides ; Aldehydes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The analysis of two metabolite groups, nucleotides and aldehydes, is necessary for assessment of oxygen radical metabolism during hypoxia and reoxygenation. Nucleotides and their derivatives were determined by HPLC using gradient elution with 10 mM NH4H2PO4 buffer containing 2 mM t-butylammoniumphosphate and acetonitrile. Aldehydes occuring after lipid peroxidation were analyzed by derivatisation to dinitrophenylhydrazones followed by TLC and HPLC separation with methanol/water on an ODS column.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02290385
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  • 3
    Electronic Resource
    Electronic Resource
    Application of reversed-phase and ion-pair HPLC for investigation of nucleotide metabolism in erythrocytes, reticulocytes and tumour cells (1988)
    Springer
    Chromatographia 25 (1988), S. 237-241 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed-phase ; Ion-pair ; Red blood cells ; Tumour cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The determination of nucleotides, nucleosides, and nucleobases was carried out in cells of different metabolic complexity: in mature and immature red blood cells, in Ehrlich ascites tumour cells from different proliferation stages, and in other tumour cells. The maturation of reticulocytes to erythrocytes is accompanied by loss of organelles and energy-requiring processes as well as the switch from aerobic to anaerobic ATP production. The profile of purine nucleotides, nucleosides, bases, and pyridine dinucleotides, by reversed-phae HPLC, shows large concentration changes during the maturation of red blood cells. The concentrations of purine mono and triphosphates are two to four times greater in reticulocytes in comparison with erythrocytes; the difference in the concentrations of nucleosides and nucleobases between reticulocytes and erythrocytes is even greater. Application of ion-pair HPLC showed that the Ehrlich ascites cells loose major portions of purine mono-, diand triphosphates between the 7th and 11th day after inoculation. Fast growing solid sarcoma tumours of rats (MV 202 Ner) contain higher amounts of nucleotides than slowly growing tumours of identical cell type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02316451
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  • 4
    Electronic Resource
    Electronic Resource
    Anion-exchange and size-exclusion chromatography of proteins in mouse ascites fluid: Changes in the protein pattern during tumour growthIn vivo (1990)
    Springer
    Chromatographia 29 (1990), S. 351-354 
    Details
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Anion exchange ; Size exclusion ; Ehrlich ascites fluid proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary During the transition of Ehrlich mouse ascites tumour cells from the proliferating to the resting state of growth a large loss of purine and pyrimidine compounds occurs. This decrease is accompanied by a change in the amount of protein in the supernate of ascites fluid, which is known from the study of the ATP-consumption during protein synthesis. The ascites fluid was investigated by anion-exchange and size-exclusion chromatography (SEC). SDS-PAGE (sodium, dodecyl sulfate-polyacrylamide gel electrophoresis) data were compared with SEC data. The total amount of protein increased by 50% between day 5 and 12 of growth. At least 5 new peaks are observed in the chromatograms of an ion-exchange separation of Ehrlich ascites fluid at day 12 postinoculation. The amounts of transferrin, albumin and IgG (immunoglobulin G) were increased to 132, 134, 157%, respectively duringin vivo growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02261302
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  • 5
    Electronic Resource
    Electronic Resource
    Fourier transform ion mobility spectrometry of barbiturates after capillary gas chromatography (1986)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 9 (1986), S. 44-48 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography, GC ; Fused silica capillary columns ; Fourier Transform Ion Mobility Spectrometry ; Ion mobility detector ; Barbiturates ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper demonstrates a novel operating mode of an ion mobility detector (IMD) for obtaining both qualitative and quantitative data after capillary gas chromatographic separation of 5,5′-disubstituted barbiturates. Using a recently developed time dispersive Fourier transform method for ion mobility spectrometry, complete ion mobility spectra could be obtained for each component in the chromatogram. This type of spectra can be used for providing qualitative information on unknown compounds or for selecting the proper detector conditions needed when operating in the continuous mobility monitoring mode.In this study each of the five barbiturates investigated produced a Fourier transformed ion mobility spectrum containing one major product ion. When drift times corresponding to those of the product ions measured in the FT mode were monitored continuously, selective chromatographic detection of the barbiturates was achieved. In one case even isomers could be differentiated based on mobility characteristics.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240090108
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