ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Hydrophobic solutes
;
Enthalpy and entropy changes
;
Eluent composition effects
;
Surface area effects
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Chromatographic retention of several nonpolar solutes by three reversed-phase liquid chromatography (RPLC) supports was measured at several temperatures with binary mixtures of methanol/water and acetonitrile/water as the mobile phase. These data were used to estimate enthalpy and entropy changes (ΔH sorp o and ΔS sorp o ) associated with solute retention. The dependence of these two thermodynamic parameters on organic solvent content (θ) in the mobile phase, solute hydrophobic surface area (HSA), and bonded n-alkyl chain length (Nc) of the RPLC support was evaluated. The differences noted in the two solvent mixtures were attributed to: (1) the manner in which solvent surface tension changes with increasing θ, and (2) the differences in the interaction of methanol and acetonitrile with the bonded alkyl chains.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02262319
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