ISSN:
1432-203X
Keywords:
axillary buds
;
callus
;
Colocasia esculenta var. esculenta
;
plant regeneration taro corm extract
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Axillary buds of taro (Colocasia esculenta var. esculenta, Araceae) cultured on half strength Murashige-Skoog medium (HMS) containing taro extract (HMSTE) and 2, 4, 5-trichlorophenoxyacetic acid produce a compact, hard, slow growing callus which is not very active morphogenetically and produces only a few plantlets. When cultured on HMSTE plus 5 mg 1−1 each of naphthaleneacetic acid and benzyl adenine (HMSNB) the buds produce a fast growing, friable and morphogenetically active callus. Meristematic regions form on the friable callus after 30 days on HMSNB. If transferred to HMSTE at this point the callus gives rise to plantlets. Addition of taro extract to the media is required for the culture of buds, induction of callus and plantlet regeneration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00232273
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