Publication Date:
2000-09-29
Description:
Cytosolic calcium oscillations control signaling in animal cells, whereas in plants their importance remains largely unknown. In wild-type Arabidopsis guard cells abscisic acid, oxidative stress, cold, and external calcium elicited cytosolic calcium oscillations of differing amplitudes and frequencies and induced stomatal closure. In guard cells of the V-ATPase mutant det3, external calcium and oxidative stress elicited prolonged calcium increases, which did not oscillate, and stomatal closure was abolished. Conversely, cold and abscisic acid elicited calcium oscillations in det3, and stomatal closure occurred normally. Moreover, in det3 guard cells, experimentally imposing external calcium-induced oscillations rescued stomatal closure. These data provide genetic evidence that stimulus-specific calcium oscillations are necessary for stomatal closure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Allen, G J -- Chu, S P -- Schumacher, K -- Shimazaki, C T -- Vafeados, D -- Kemper, A -- Hawke, S D -- Tallman, G -- Tsien, R Y -- Harper, J F -- Chory, J -- Schroeder, J I -- R01 GM60396-01/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2000 Sep 29;289(5488):2338-42.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cell and Developmental Biology Section, Division of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0116, USA. gallen@biomail.ucsd.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11009417" target="_blank"〉PubMed〈/a〉
Keywords:
Abscisic Acid/pharmacology
;
Arabidopsis/cytology/genetics/*physiology
;
Calcium/metabolism
;
*Calcium Signaling
;
Cell Membrane/metabolism
;
Cold Temperature
;
Endoplasmic Reticulum/metabolism
;
Genes, Plant
;
Hydrogen Peroxide/pharmacology
;
Membrane Potentials
;
Mutation
;
Oxidative Stress
;
Plant Leaves/cytology/*physiology
;
Potassium/metabolism
;
Proton-Translocating ATPases/genetics/metabolism
;
*Vacuolar Proton-Translocating ATPases
;
Vacuoles/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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