ISSN:
1432-072X
Keywords:
Cobalt
;
Iron
;
Nickel
;
Biotin
;
Methanococcoides methylutens
;
Trace elements
;
Vitamins
;
Methanogens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Trace organic nutrient and metal requirements for the growth of Methanococcoides methylutens strain TMA-10 were determined in defined medium that contained trimethylamine as the substrate. Biotin was the only organic supplement required in place of yeast extract, Trypticase or a mixture of 8 B-vitamins. Fe an Ni were required for growth and low concentrations of Fe2+ (〈5 μM) and Ni2+ (〈0.25 μM) provided limited growth. In the absence of added Co the growth rate was reduced by 94% and growth was limited at concentrations below 0.1 μM. Stimulation of growth by Se, Mo, B, Al, Zn, Mn or Cu could not be demonstrated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00447058
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