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  • 1
    Electronic Resource
    Electronic Resource
    RAPDs identify varietal misclassification and regional divergence in cranberry [Vaccinium macrocarpon (Ait.) Pursh] (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 1004-1010 
    Details
    ISSN: 1432-2242
    Keywords: Cranberry ; Vaccinium macrocarpon RAPDs ; Variety identification ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The majority of cultivated cranberry varieties were selected from native populations in the 1800s and early 1900s from sites in Massachusetts, New Jersey, and Wisconsin. Since their initial selections 100–150 years ago, varietal identities have become increasingly confused; primarily the result of there being a paucity of qualitative markers to effectively distinguish among varieties. Random amplified polymorphic DNA (RAPD) technology has the potential for allowing a more definitive classification of varieties and was used in this study to characterize 22 cranberry varieties. Twenty-two decamer primers amplified 162 scorable DNA fragments, of which 66 (41%) were polymorphic. On the basis of these 66 silver-stained RAPDs (ssRAPDs), 17 unique profiles were identified rather than the expected 22. Fourteen varieties had unique ssRAPD profiles, while the remaining 8 were represented by 3 ssRAPD profiles. Permuational analyses of the data suggest that the observed ssRAPD profile duplications are examples of varietal misclassification. Further analyses identified 2 ssRAPD markers that were found only in Eastern varieties (from Mass. and N. J.) and not in Wisconsin varieties. With varieties differing on average by 22 bands, ssRAPDs are shown to be effective in varietal identification and the assessment of genetic diversity in cranberry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220808
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for RAPD heteroduplex formation in cranberry: implications for pedigree and genetic-relatedness studies and a source of co-dominant RAPD markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 840-849 
    Details
    ISSN: 1432-2242
    Keywords: Heteroduplex ; RAPD ; Co-dominant markers ; Silver stain ; Genetic relatedness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Silver-stained random amplified polymorphic DNA (ssRAPD) markers have been identified that are always jointly present or absent in the ssRAPD profiles of cranberry varieties. On the basis of segregation data and the ability to re-create these “associated ssRAPDs” through the intermixing of amplified DNA from individuals lacking them, five of the six pairs of associated ssRAPDs analyzed were shown to be consistent with heteroduplex molecules. Heteroduplexes are “hybrid”” double-stranded DNAs that are formed following the polymerase chain reaction (PCR) amplification of two DNA segments that have a high degree of homology to one another, yet differ in their nucleotide sequences as a result of base pair deletions, additions, or substitutions. Three of the five putative heteroduplex systems identified are consistent with a one locus, two-allele heteroduplex model. The remaining two systems appeared to be multi-allelic, involving interactions among three and four alleles, respectively. RAPD heteroduplex formation has the potential to confound genetic relatedness and pedigree studies. Heterozygous individuals exhibit heteroduplex RAPDs not seen in either of the two homozygote classes. Genetic estimates under such a circumstance would “inflate” the differences between the heterozygote and the homozygote classes. Heteroduplex formation is also a mechanism for the presence of non-parental RAPDs in progeny of parents homozygous for alternate alleles. While this class of molecular markers can confound RAPD analyses, they also offer a source of co-dominant RAPD markers, which are of value in genetic relatedness estimates and as markers for studying breeding behavior.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221896
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