ISSN:
1617-4623
Keywords:
Cloning
;
Xanthomonas
;
Protease
;
Pathogenicity
;
Brassica
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The recombinant plasmid pIJ3070 isolated from a genomic library of Xanthomonas campestris pv. campestris constructed in the conjugal cosmid pLAFR3 contains protease gene(s) which can be expressed in Escherichia coli. Tn5 mutagenesis and subcloning revealed that the protease structural gene(s) is(are) located in a ca. 10 kb EcoRI fragment. Several protease-minus mutants of X. c. campestris were obtained by Tn5 mutagenesis of pIJ3070 and marker exchange techniques. Studies of pathogenicity of these Tn5 mutants showed that the protease is not critically important for the pathogenicity of X. c. campestris on turnip plants but may play a minor role in disease development.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327195
Permalink