Publication Date:
2002-09-07
Description:
Cells have evolved multiple mechanisms to inhibit viral replication. To identify previously unknown antiviral activities, we screened mammalian complementary DNA (cDNA) libraries for genes that prevent infection by a genetically marked retrovirus. Virus-resistant cells were selected from pools of transduced clones, and an active antiviral cDNA was recovered. The gene encodes a CCCH-type zinc finger protein designated ZAP. Expression of the gene caused a profound and specific loss of viral messenger RNAs (mRNAs) from the cytoplasm without affecting the levels of nuclear mRNAs. The finding suggests the existence of a previously unknown machinery for the inhibition of virus replication, targeting a step in viral gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gao, Guangxia -- Guo, Xuemin -- Goff, Stephen P -- CA 30488/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 2002 Sep 6;297(5587):1703-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12215647" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antiviral Agents/chemistry/*genetics/isolation & purification/physiology
;
Carrier Proteins/chemistry/*genetics/isolation & purification/physiology
;
Cell Line
;
Cloning, Molecular
;
Gene Library
;
Genetic Vectors/genetics
;
Open Reading Frames
;
Polymerase Chain Reaction
;
RNA, Viral/*biosynthesis
;
Rats
;
Retroviridae/*genetics/immunology
;
Tissue Distribution
;
Virus Replication
;
*Zinc Fingers
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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