Publication Date:
1996-02-02
Description:
Targeted integration of exogenous DNA into the genome of malaria parasites will allow their phenotype to be modulated by means of gene disruption or the stable expression of foreign and mutated genes. Described here is the site-specific integration through reciprocal exchange, and subsequent expression, of a selectable marker gene into the genome of the pathogenic, bloodstage forms of the rodent malaria parasite Plasmodium berghei. Stable integration of a single copy of the marker gene (retained for more than 70 generations in the absence of drug pressure) into a nontranscribed subtelomeric repeat array of different chromosomes was observed. Expression of the gene within the subtelomeres indicated that the previously recorded absence of transcription in these regions could be due to a corresponding absence of genes rather than active silencing mechanisms.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉van Dijk, M R -- Janse, C J -- Waters, A P -- New York, N.Y. -- Science. 1996 Feb 2;271(5249):662-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Parasitology, University of Leiden, Netherlands.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8571132" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antimalarials/pharmacology
;
Chromosomes/genetics
;
Drug Resistance
;
Electroporation
;
Gene Expression
;
*Genes, Protozoan
;
Genetic Vectors
;
Multienzyme Complexes/*genetics
;
Plasmids
;
Plasmodium berghei/drug effects/enzymology/*genetics
;
Pyrimethamine/pharmacology
;
Repetitive Sequences, Nucleic Acid
;
Telomere/*genetics
;
Tetrahydrofolate Dehydrogenase/*genetics
;
Thymidylate Synthase/*genetics
;
*Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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