Publication Date:
2010-04-27
Description:
Induced pluripotent stem cells (iPSCs) have been generated by enforced expression of defined sets of transcription factors in somatic cells. It remains controversial whether iPSCs are molecularly and functionally equivalent to blastocyst-derived embryonic stem (ES) cells. By comparing genetically identical mouse ES cells and iPSCs, we show here that their overall messenger RNA and microRNA expression patterns are indistinguishable with the exception of a few transcripts encoded within the imprinted Dlk1-Dio3 gene cluster on chromosome 12qF1, which were aberrantly silenced in most of the iPSC clones. Consistent with a developmental role of the Dlk1-Dio3 gene cluster, these iPSC clones contributed poorly to chimaeras and failed to support the development of entirely iPSC-derived animals ('all-iPSC mice'). In contrast, iPSC clones with normal expression of the Dlk1-Dio3 cluster contributed to high-grade chimaeras and generated viable all-iPSC mice. Notably, treatment of an iPSC clone that had silenced Dlk1-Dio3 with a histone deacetylase inhibitor reactivated the locus and rescued its ability to support full-term development of all-iPSC mice. Thus, the expression state of a single imprinted gene cluster seems to distinguish most murine iPSCs from ES cells and allows for the prospective identification of iPSC clones that have the full development potential of ES cells.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987905/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3987905/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stadtfeld, Matthias -- Apostolou, Effie -- Akutsu, Hidenori -- Fukuda, Atsushi -- Follett, Patricia -- Natesan, Sridaran -- Kono, Tomohiro -- Shioda, Toshi -- Hochedlinger, Konrad -- DP2 OD003266/OD/NIH HHS/ -- Howard Hughes Medical Institute/ -- England -- Nature. 2010 May 13;465(7295):175-81. doi: 10.1038/nature09017. Epub 2010 Apr 25.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Howard Hughes Medical Institute at Massachusetts General Hospital, Center for Regenerative Medicine; Harvard Stem Cell Institute, 185 Cambridge Street, Boston, Massachusetts 02114, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20418860" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
Chromosomes, Mammalian/*genetics
;
Embryonic Stem Cells/metabolism
;
Epigenesis, Genetic/genetics
;
Female
;
Fibroblasts
;
*Gene Expression Profiling
;
*Gene Silencing
;
Genomic Imprinting/*genetics
;
Intercellular Signaling Peptides and Proteins/genetics
;
Mice
;
MicroRNAs/genetics/metabolism
;
Multigene Family/genetics
;
Nuclear Proteins/genetics
;
Pluripotent Stem Cells/cytology/*metabolism
;
Proteins/genetics
;
RNA, Long Noncoding
;
RNA, Messenger/genetics/metabolism
;
Transcription, Genetic/genetics
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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