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  • Oryza  (2)
  • Tobacco  (2)
  • Chloroplast DNA  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Cellular dissection of the degradation pattern of cortical cell death during aerenchyma formation of rice roots (1998)
    Springer
    Planta 204 (1998), S. 277-287 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Aerenchyma ; Cell death ; Oryza ; Plasmodesma ; Root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Cellular events which occur prior to cell collapse were examined in the root cortex of rice (Oryza sativa L.) during aerenchyma formation. Cell collapse started at a specific position in the mid cortex. These cells were distinct in shape from those located towards the periphery. Furthermore, cell collapse was preceded by acidification and the loss of plasma-membrane integrity in cells of the mid cortex. Subsequent death of neighboring cells followed a radial path. Microinjection of molecules of different sizes conjugated with fluorescein isothiocyanate (FITC) showed a molecular exclusion limit of between 9.3 and 19.6 kDa in the root cortex. Furthermore, large molecules, i.e. those around 9.3 kDa, were predominantly transferred in a radial direction, which coincided with the path of sequential cell death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050257
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  • 2
    Electronic Resource
    Electronic Resource
    Cortical cell death, cell proliferation, macromolecular movements and rTip1 expression pattern in roots of rice (Oryza sativa L.) under NaCl stress (1999)
    Springer
    Planta 207 (1999), S. 354-361 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Aerenchyma ; Cell death ; Oryza ; Root ; Stress (NaCl)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The mode of action of NaCl in terms of cell proliferation and cell death was examined in seminal roots of rice plants (Oryza sativa L.). Salt/sodium chloride was inhibitory to cell number increase and to cell death in cortical tissue, whereas final cortical cell size was the same as in control roots that were not exposed to NaCl. It seems that NaCl may stimulate the transition phase from cell division to cell elongation. Further analysis of the role of NaCl in the suppression of cortical cell death was confined to a delay in the early stage of cell collapse, which was caused by tonoplast disruption, and plasma-membrane destruction. Sodium chloride did not have any effect on the cell-to-cell movement of macromolecules in the root cortex. In-situ hybridization studies indicated that expression of the gene for tonoplast intrinsic protein (rTip1) was localized predominantly in the epidermal and exodermal cells as well as in metaxylem cells in seminal roots. Upon NaCl treatment, the intensity of rTip1 gene expression was raised in the cortical parenchyma, suggesting that salt plays a role in the rapid onset of cell elongation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004250050492
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of nuclear and cytoplasmic information in the progeny of a somatic hybrid between male sterile Nicotiana tabacum and N. glutinosa (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 95-100 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana somatic hybrid ; Ribosomal RNA gene ; Chromosome ; Chloroplast DNA ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several nuclear and cytoplasmic characters of the back-crossed progeny of a somatic hybrid between male sterile Nicotiana tabacum (N. debneyi cytoplasm) and N. glutinosa have been analysed. Progeny were obtained by repeated back-crossing of a somatic hybrid with pollen from either N. tabacum or N. glutinosa. Nuclear ribosomal RNA genes (rDNA) were found to be a reliable marker to determine the constitution of nuclear genomes in the progeny. The progeny obtained by back-crossing with N. tabacum pollen maintained uniformity in leaf morphology. On the other hand, variation in leaf morphology was observed in the second back-cross population obtained with N. glutinosa pollen. This may be due to a variable contribution of N. tabacum chromosomes. Segregation of rDNA was also found in individuals of the same back-crossed progeny, but was not related to the chromosome number. The stable inheritance of chloroplast DNA in the back-crossed generation was confirmed regardless of the type of pollen donor. Male sterility was consistently maintained throughout several generations, suggesting that the nuclear genome of either N. tabacum or N. glutinosa does not influence the expression of cytoplasmic male sterility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252321
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  • 4
    Electronic Resource
    Electronic Resource
    Inheritance and structure of foreign DNA in progenies of transgenic tobacco obtained by direct gene transfer (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 161-164 
    Details
    ISSN: 1432-2242
    Keywords: Tobacco ; Direct DNA transformation ; Insertion site ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One of the transformed tobacco plants obtained by direct DNA transformation possessed two marker genes, a chimeric aminoglycoside phosphotransferase and nopaline synthase genes. Selfed progenies of this plant (T3-d) showed stable inheritance of these two genes. The minimum size of foreign DNA integrated into tobacco genome was estimated to be 5.4 kbp. A deleted nopaline synthase gene co-existed with an intact gene. The linkage analysis indicated that two transformants, T1-b and T3-c, possessed foreign DNA inserted in different chromosomes or in different sites of the same chromosome that recombine freely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257840
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  • 5
    Electronic Resource
    Electronic Resource
    Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusion (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 69-72 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast fusion ; Cytoplasmic male sterility ; Tobacco ; Fraction I protein ; TMV resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts derived from suspension cultured cells of cytoplasmic male sterile Nicotiana tabacum (N. debneyi cytoplasm) and of fertile N. glutinosa were fused with the aid of polyethylene glycol (PEG). Out of 1,089 colonies developed from PEG-treated protoplasts, 29 restored whole plants. A somatic hybrid plant was selected on the basis of isoelectrofocusing analysis of Fraction I protein in leaves of regenerated plants. A newly created hybrid contained small subunits of both parents but only a N. glutinosa type large subunit. Male sterile character was conserved in a hybrid plant while leaf morphology was intermediate between the parents. By tobacco mosaic virus infection tests, the hybrid's leaves showed resistant symptoms, hypersensitive local lesions, which were due to N. glutinosa nuclear genome expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261514
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