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  • Chlorobenzoate  (1)
  • Energy conservation  (1)
  • Fe3+Mn4+ reduction  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 149 (1987), S. 102-105 
    ISSN: 1432-072X
    Schlagwort(e): Reductive dehalogenation ; Chlorobenzoate ; Energy conservation ; Chemotrophy ; Growth yield ; Interspecies hydrogen transfer
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The microbially mediated reductive dehalogenation of aromatic compounds is potentially important in removal of chlorinated aromatic compounds from the environment. Thermodynamic data are presented which show that the reductive dechlorination of 3-chlorobenzoate to benzoate is exergonic, which led to the hypothesis that reductive elimination of chlorine from 3-chlorobenzoate yields biologically useful energy. In the present paper this hypothesis is tested. Experimental data were obtained with a defined 3-chlorobenzoate degrading methanogenic consortium. These data showed that (i) the molar growth yield of a defined 3-chlorobenzoate degrading consortium increased from 4.9 g protein per mol benzoate metabolized to 6.8 g protein per mol 3-chlorobenzoate when 3-chlorobenzoate replaced benzoate as energy source, and that (ii) the ATP level in starved consortium cells was twice as high when the cells were fed 3-chlorobenzoate than when fed benzoate. These observations show that the electrochemical potential between the redox partners of the H+/H2 (electron-donating) and 3-chlorobenzoate/benzoate (electron-accepting) couples is a potential source of energy and are consistent with the hypothesis that reductive dechlorination of aromatic compounds is coupled to a novel type of microbial chemotrophy.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 76 (1984), S. 197-212 
    ISSN: 1573-5036
    Schlagwort(e): Aggregate oxygen profile ; Anaerobic radii ; Anaerobiosis ; Anoxia ; Aromatic ring cleavage ; Clostridium ; Dechlorination ; Denitrification ; Denitrification pathway hypothesis ; Fermentation ; Fe3+Mn4+ reduction ; Methanogenic microorganisms ; 13N15N N−N bond N2O ; NO 3 − respiration/dissimilatory reduction ; O2 consumption ; O2 diffusion coefficient ; O2 micro electrode ; Pesticides ; Reduction NO 3 − NO 2 − SO 4 2− CO2H+ ; Soils flooded poorly and well drained ; Sulphate ; Xenobiotics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Anaerobic conditions in soil affect plant productivity as well as organic matter and nutrient dynamics. Anaerobic processes often dominate biological and chemical features of flooded and poorly drained soils but in well-drained soils, anoxia is restricted to small zones and to limited periods. The anaerobic processes listed according to their approximate sequence of occurrence as the redox decreases are: Fe3+ and Mn4+ reduction, denitrification, fermentation, nitrate respiration, dissimilatory nitrate reduction to ammonium, sulfate reduction, carbon dioxide reduction, acetate splitting, and proton reduction. Two of the anaerobic processes, denitrification and fermentation of pollutant chemicals have been studied and recent results are summarized here. We describe the measurement of denitrification using a recirculating atmosphere of acetylene, provide quantitative information on the effect of moisture and oxygen on denitrification, and report on the oxygen concentration within soil aggregates measured by oxygen microelectrodes. The current hypotheses for the pathway leading from nitrate to the N−N bond and N2O are also presented. Recent work in our laboratory has shown a new reaction for the metabolism of some chlorinated organic chemicals. In this reductive dehalogenation, the ring Cl is replaced by a proton. An unusual organism which carried out this reaction has been enriched and isolated on 3-chlorobenzoate. These anaerobic reactions may be of further use in pollutant removal. With these new techniques and knowledge available, it is now possible to gain a better understanding of the biochemistry, physiology, ecology and diversity of the anaerobic organisms and their processes in soil.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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