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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of cDNA clones encoding the 17.9 and 8.1 kDa subunits of Photosystem I from Chlamydomonas reinhardtii (1989)
    Springer
    Plant molecular biology 12 (1989), S. 463-474 
    Details
    ISSN: 1573-5028
    Keywords: cDNA sequence ; Chlamydomonas reinhardtii ; chloroplast ; nuclear-encoded subunits ; Photosystem I ; transit peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA clones encoding two Photosystem I subunits of Chlamydomonas reinhardtii with apparent molecular masses of 18 and 11 kDa (thylakoid polypeptides 21 and 30; P21 and P30 respectively) were isolated using oligonucleotides, the sequences of which were deduced from the N-terminal amino acid sequences of the proteins. The cDNAs were sequenced and used to probe Southern and Northern blots. The Southern blot analysis indicates that both proteins are encoded by single-copy genes. The mRNA sizes of the two components are 1400 and 740 nucleotides, respectively. Comparison between the open reading frames of the cDNAs and the N-terminal amino acid sequences of the proteins indicates that the molecular masses of the mature proteins are 17.9 (P21) and 8.1 kDa (P30). Analysis of the deduced protein sequences predicts that both subunits are extrinsic membrane proteins with net positive charges. The amino acid sequences of the transit peptides suggest that P21 and P30 are routed towards the lumenal and stromal sides of the thylakoid membranes, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017585
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of the genes of the OEE1 and OEE3 proteins of the photosystem II complex from Chlamydomonas reinhardtii (1989)
    Springer
    Plant molecular biology 12 (1989), S. 683-693 
    Details
    ISSN: 1573-5028
    Keywords: Chlamydomonas reinhardtii ; chloroplast ; oxygen evolving complex ; photosystem II ; transit peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sequences of the nuclear genes of the 33 kDa (OEE1) and the 16 kDa (OEE3) polypeptides of the oxygen evolving complex of Chlamydomonas reinhardtii have been established. Comparison between the OEE1 protein sequences of C. reinhardtii and higher plants and cyanobacteria reveals 67 and 47% homology. In contrast, C. reinhardtii and higher plants have only 28% overall homology for OEE3 which is mostly limited to the central portion of the protein. The transit peptides of the C. reinhardtii proteins consist of 52 (OEE1) and, most likely, 51 (OEE1) amino acids. They have a basic amino terminal region and, at least in the case of OEE1, a hydrophobic segment at their carboxy terminal end typical of thylakoid lumen proteins. Comparison of the genomic and cDNA clones indicates that the OEE1 and OEE3 genes contain five and four introns, respectively, some of which are located within the coding sequences of the transit peptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044159
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of cDNA clones encoding Photosystem I subunits with molecular masses 11.0, 10.0 and 8.4 kDa from Chlamydomonas reinhardtii (1989)
    Springer
    Molecular genetics and genomics 219 (1989), S. 137-144 
    Details
    ISSN: 1617-4623
    Keywords: cDNA sequence ; Chlamydomonas reinhardtii ; Chloroplast ; Nuclear-encoded subunits ; Photosystem I
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones encoding three photosystem I subunits of Chlamydomonas reinhardtii with apparent molecular masses 13, 5 and 3 kDa (thylakoid polypeptides 28, 35 and 37; P28, P35 and P37, respectively) were isolated using gene specific oligonucleotides as probes. The sequences of these oligonucleotides were deduced from the N-terminal amino acid sequences of the proteins. The cDNAs were sequenced and used to probe Southern and Northern blots. The Southern blot analysis indicates that the proteins are encoded by single-copy genes. The mRNA sizes of the three components are 960 (P28), 1120 (P35) and 790 (P37) nucleotides. Comparison between the open reading frames of the cDNAs and the N-terminal amino acid sequences of the proteins indicates that the nascent polypeptides possess N-terminal transit sequences that are removed to give mature proteins of 11.0 (P28), 10.0 (P35) and 8.4 (P37) kDa. Analysis of the deduced protein sequences suggests that P28 and P35 are extrinsic membrane proteins and that P37 spans the thylakoid membrane. All three proteins have short transit peptides that probably route them to the stromal side of the thylakoid membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261169
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