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  • Chlamydomonas  (2)
  • Flagellar regeneration  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A gene essential for viability and flagellar regeneration maps to the uni linkage group of Chlamydomonas reinhardtii (1989)
    Springer
    Current genetics 15 (1989), S. 377-384 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas reinhardtii ; Flagellar regeneration ; ts Mutants ; uni Linkage group
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated a mutant of Chlamydomonas reinhardtii that is both temperature sensitive for viability and temperature sensitive for flagellar regeneration. The mutation (designated tnr1, for temperature-sensitive nonregenerator) has been genetically mapped to a position near uni1 on the uni linkage group (ULG), an unusual genetically circular linkage group consisting primarily of mutations affecting flagellar assembly or function. tnr1 is the first essential gene identified on this linkage group, and is one of the few essential genes affecting flagellar function identified to date. We also find that tnr1 cells are not defective for induction of new tubulin transcripts or protein synthesis during flagellar regeneration at the nonpermissive temperature, and that at least a portion of the unassembled pool of flagellar proteins in mutant cells is assembly-competent at the nonpermissive temperature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419919
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  • 2
    Electronic Resource
    Electronic Resource
    Extensive restriction fragment length polymorphisms in a new isolate ofChlamydomonas reinhardtii (1988)
    Springer
    Current genetics 13 (1988), S. 503-508 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new field isolate of the unicellular green algaChlamydomonas reinhardtii with useful properties for restriction fragment length polymorphism mapping is described in this report. The isolate, S1-D2 (mating type-), was the only strain found among 24Chlamydomonas isolates taken from many locations which was interfertile with laboratory strains ofC. reinhardtii. It mates at high efficiency, giving tetrads with excellent viability. Using cloned probes for both nuclear and chloroplast genes, we have found numerous restriction fragment length polymorphisms between Sl-D2 and laboratory strains ofC. reinhardtii.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02427756
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of dominant, pleiotropic drug-resistance mutants in Chlamydomonas reinhardtii (1989)
    Springer
    Current genetics 15 (1989), S. 443-452 
    Details
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Drug resistance ; Pleiotropy ; Dominance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three independent pleiotropic drug-resistance (pdr) mutants were isolated by selecting for resistance to the anti-microtubule herbicides amiprophos-methyl (APM) and oryzalin (ORY). These three mutants and a previously isolated mutant, ani1 (anisomycin resistance), were semi-dominant in heterozygous diploids, and they displayed varying degrees of resistance to structurally and functionally unrelated inhibitors such as cycloheximide, cryptopleurine, emetine, atrazine, and nonidet P-40. Linkage analysis and genetic mapping suggested that three of the four mutants, including ani1, define a single locus, here named pdr1. The fourth mutant defined a new locus, pdr2, which is located on the left arm of linkage group VI. One pdr1 mutant exhibited unusual genetic interactions, including enhanced ts-lethality and synergistic increases in drug resistance, when combined with pdr2-1 and with herbicide-resistant alleles of three other genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376802
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