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  • 1
    Electronic Resource
    Electronic Resource
    Production and characterization of a plant α-hydroxynitrile lyase in Escherichia coli (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 53 (1997), S. 332-338 
    Details
    ISSN: 0006-3592
    Keywords: α-hydroxynitrile lyase ; cassava ; cyanogenesis ; cyanohydrin ; Escherichia coli expression vector ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The coding sequence of the cyanogenic α-hydroxynitrile lyase gene of Manihot esculenta Crantz (cassava) was cloned in the plasmid vector pMal-c2 and expressed in Escherichia coli strain JM105. DNA sequencing showed that the recombinant plasmid contained the same sequence as the cDNA clone pHNL10. Peptide sequencing of the recombinant protein showed that the N-terminus was heterogeneous, with either four or six additional amino acid residues compared with the native protein. Circular dichroism spectra indicated similar secondary structure contents for both proteins. Enzyme assays showed that specific activity of native and recombinant proteins were 0.24 and 0.26 mmol CN-/mg/min, respectively; that both proteins had optimal activity at 40°C and pH 5.5; and that both proteins were inhibited by the serine protease inhibitor phenyl-methane sulfonyl flouride (PMSF). Isoelectric focusing of native and recombinant protein revealed multiple isoforms for both proteins; the recombinant protein had a more basic mean isoelectric point (pl) (5.1) than the native protein (4.5). © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 332-338, 1997.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Some thermal studies on poly(ethylene terephthalate) (1964)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 8 (1964), S. 1541-1548 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Differential thermal analysis (DTA) and thermogravimetric analysis (TGA) of poly(ethylene terephthalate) has been carried out on samples of amorphous, cold-drawn, heat-crystallized, and acetone-crystallized polymer. From DTA it appears that for amorphous material a prolonged “secondary” crystallization process follows a primary process in the region of 140°C. For cold-drawn polymer, heat treatment does not reflect differences observed in previous x-ray diffraction studies. The TGA gives evidence that a high temperature endothermic peak observed in DTA may not be directly associated with depolymerization. With acetone-crystallized material a high degree of acetone retention is detected.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1964.070080404
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  • 3
    Electronic Resource
    Electronic Resource
    Interfacial behaviour of LIX 63 and The Surface Kinetics of Copper Extraction (1990)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 332 (1990), S. 277-292 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Different adsorption isotherms such as the Szyszkowski, Temkin, Frumkin, polynomial and spline functions were explore as fits to the experimental interfacial tension isotherms for LIX-63-hexane/water and LIX-63-toluene/water systems and then the surface excess isotherms were computed. A good match was obtained. The surface excess isotherms change in a similar way as those reported previously for 2-hydroxy-5-alkylbenzophenone oximes.Kinetic equations were derived for various versions of the extraction mechanism at the molecular interface, and the interfacial tension data were then used to estimate reaction orders against hydroxyoxime concentrations assuming that the process occurs at the interface of molecular dimensions. A reaction order equal one was obtained in the case of the formation of the stable 2:1 complex from the surface adsorbed intermediate 1:1 complex with a hydroxyoxime molecule held in the aqueous sublayer. This is the slowest step in the overall process. Hydroxyoxime association has only a small effect upon the predicted reaction orders and can be neglected.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19903320302
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