ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Analytical capillary isotachophoresis has been applied as an analytical tool for the assay of the microsomal enzyme UDP-glucuronyltransferase, which is an important system for the detoxification of drugs and many endogenous and exogenous toxins. The substrate donor molecule UDP-glucuronic acid, various products of hydrolytic cleavage, and the products of glucuronidation β-glucuronides, can be assayed in a single run. Furthermore, the glucuronidation of mixtures of acceptors can be monitored simultaneously, since the various β-glucuronides are separated from one another. This method offers a more comprehensive assay of glucuronidation reactions than has been possible using previously published procedures.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150020104
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