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  • Chemistry  (23)
  • Superfluidity and superconductivity  (6)
  • Surface physics, nanoscale physics, low-dimensional systems  (3)
  • Tobacco  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Expression pattern of tobacco cyclin genes (1997)
    Springer
    Plant cell reports 16 (1997), S. 368-372 
    Details
    ISSN: 1432-203X
    Keywords: Key words Mitotic cyclin ; In situ hybridization ; Cell cycle ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously isolated three cDNA clones (Ntcyc25, Ntcyc27, Ntcyc29) encoding mitotic cyclins from tobacco (Nicotiana tabacum), and in this report we describe the expression patterns of these genes. RNA gel-blot analysis showed that the mitotic cyclin genes were expressed predominantly in actively dividing meristematic tissues such as the shoot apex and young developing leaves. In situ hybridization analysis indicated that while the Ntcyc29 gene was expressed consistently at low levels in shoot apex, but its transcripts were found at relatively high levels in the flower bud, indicating the possibility that the Ntcyc29 product plays a role in flower development. The Ntcyc25 and Ntcyc29 genes were expressed differentially in the root apex during the cell cycle, confirming the result obtained using synchronized-cultured tobacco cells. These results suggest that the transcriptionally regulated cyclin genes may be important in controlling cell division in tobacco.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146775
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  • 2
    Electronic Resource
    Electronic Resource
    Two types of heat shock factors in cultured tobacco cells (2000)
    Springer
    Plant cell reports 19 (2000), S. 414-420 
    Details
    ISSN: 1432-203X
    Keywords: Key words Arabidopsis thaliana ; cDNA cloning ; DNA-binding activity ; Heat shock factor ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have isolated two types of complementary DNA (cDNA) sequences showing high similarity with the DNA-binding domain in heat shock factors (HSFs) from a cDNA library of tobacco suspension-cultured cells (Nicotiana tabacum L. cv. BY2). These two genes, NtHSF1 and NtHSF2, showed low similarity (37%) with each other. Both contained the typical conserved regions of HSFs (DNA-binding domains, leucine zipper repeats, nuclear localization signals, and tryptophan repeats). Transcripts of NtHSF1 and NtHSF2 were detected even at the normal temperature. The recombinant NtHSF1 and NtHSF2 proteins expressed in Escherichia coli bound specifically to a 183-bp DNA fragment of the Arabidopsis thaliana HSP18.2 promoter containing three sets of head-to-head and tail-to-tail repeats of the conserved pentamer unit (nGAAnnTTCnnGAAn).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050749
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  • 3
    Electronic Resource
    Electronic Resource
    Expression pattern of tobacco cyclin genes (1997)
    Springer
    Plant cell reports 16 (1997), S. 368-372 
    Details
    ISSN: 1432-203X
    Keywords: Mitotic cyclin ; In situ hybridization ; Cell cycle ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously isolated three cDNA clones (Ntcyc25, Ntcyc27, Ntcyc29) encoding mitotic cyclins from tobacco (Nicotiana tabacum), and in this report we describe the expression patterns of these genes. RNA gelblot analysis showed that the mitotic cyclin genes were expressed predominantly in actively dividing meristematic tissues such as the shoot apex and young developing leaves. In situ hybridization analysis indicated that while theNtcyc29 gene was expressed consistently at low levels in shoot apex, but its transcripts were found at relatively high levels in the flower bud, indicating the possibility that theNtcyc29 product plays a role in flower development. TheNtcyc25 andNtcyc29 genes were expressed differentially in the root apex during the cell cycle, confirming the result obtained using synchronized-cultured tobacco cells. These results suggest that the transcriptionally regulated cyclin genes may be important in controlling cell division in tobacco.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146775
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  • 4
    Electronic Resource
    Electronic Resource
    Mammalian Alkaloids: O-Methylation of (±)-Norcoclaurine-1-carboxylic Acid and Related Isoquinolines Including (S)- and (R)-Norcoclaurine with 14C-Labeled S-Adenosyl-L-Methionine in Presence of Mammalian Catechol O-Methyltransferase (1990)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 73 (1990), S. 426-432 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: (±)-Norcoclaurine-1-carboxylic acid (5) and the derived dihydroisoquinolinone 6 (present as quinonemethide 6a at pH 7) afforded, on methylation with 14C-labeled S-adenosyl-L-methionine in the presence of mammalian catechol O-methyltransferase, exclusively the 7-O-methylated congeners 7 and 9, respectively. High stereoselectivity of the O-methylation was observed with (-)-(S)- and (+)-(R)-norcoclaurine (2a and 2b, resp.), affording 80% of 6-O-methylated isoquinoline 12 and 20% of the 7-O-methylated isomer 11 from 2a, and the reversed proportion of 12 and 11 from 2b. Synthesis of the reference amino acid 8 was achieved by Pictet-Spengler condensation of O-benzyl-protected dopamine 17 with benzyl-protected keto acid 20 (→ 21) followed by methylation with diazomethane (→22 + 23) and removal of the protecting groups by acid hydrolysis. It is considered unlikely that amino acids such as 5 constitute important precursors in the biosynthesis of isoquinolines related to reticuline.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19900730222
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  • 5
    Electronic Resource
    Electronic Resource
    Thermal properties of poly(2-carboxyethylmethylsiloxane)-poly(dimethylsiloxane) complexes (1986)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 187 (1986), S. 1169-1174 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The thermal properties of complexes between poly(2-carboxyethylmethylsiloxane) and poly-(dimethylsiloxane) are investigated. The results of DSC measurements indicate that the first-order transition of the complexes is affected by the amount of poly(dimethylsiloxane) in the complex. From dynamic viscoelasticity measurements, it is inferred that the complex exhibits entropy elasticity due to a network structure maintained by hydrogen bonds.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1986.021870514
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of various apolar groups in polycationic polyorganosiloxanes upon polyion complexation with poly(methacrylic acid) (1986)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 187 (1986), S. 1159-1167 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Polyion complexes are formed from poly(methacrylic acid) and polycationic polyorganosiloxanes modified with various apolar groups in the main chain or in the side chains. Potentiometric titration measurements with solutions of the complexes indicate that the chemical structure of the composed polycation influences complexation in aqueous solution. From a binding study with methyl orange, it is seen that the electrostatic interaction of the polyion complexes is enhanced by an increase of the hydrophobicity in the composed polycations. In water/ethanol solutions, however, such a tendency is not observed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1986.021870513
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  • 7
    Electronic Resource
    Electronic Resource
    Stereoselective Synthesis of Enantiomerically Pure β-Fluoroalkyl γ-Butyrolactones by Sulfoxide-Directed Lactonization (1999)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1999 (1999), S. 111-115 
    Details
    ISSN: 1434-193X
    Keywords: Fluorine ; Lactones ; Annulation ; Ketene ; Sulfoxides ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: -Enantiomerically pure α,α-dichloro β-fluoroalkyl γ-p-tolylthio γ-butyrolactones trans-6a-c have been obtained with excellent stereocontrol (〉 98:2) and enantiomeric purity (〉 98:2) by sulfoxide-directed lactonization (Marino's annu-lation reaction) of β-fluoroalkyl vinyl sulfoxides (R)-(E)-5a-c with dichloroketene. Highly chemoselective dechlorination and desulfurization reactions performed on trans-6c efficiently provided the β-chlorodifluoromethyl γ-butyrolactone (S)-8c, the absolute stereochemistry of which was determined by X-ray diffraction analysis of its γ-p-tolylthio precursor (2R,3S)-7c.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Synthesis of 2-Fluoro Analogues of Frontalin (2000)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 2000 (2000), S. 1387-1389 
    Details
    ISSN: 1434-193X
    Keywords: Natural products ; Epoxidations ; Asymmetric synthesis ; Fluorine ; Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The synthesis of enantiomerically and diastereomerically pure (-)-(1R,2R,5R)- and (-)-(1R,2S,5R)-2-fluoro frontalin (7) starting from (+)-(1S)-menthyl-(R)-toluene-4-sulfinate, methylmagnesium bromide, methyl fluoroacetate, 4-pentenyl bromide and diazomethane is described. The absolute stereochemistry was unambiguously determined by X-ray analysis of (+)-(1S,2R,5S,RS)-5, an intermediate in the synthesis of the enantiomeric (+)-(1S,2R,5S)-2-fluoro frontalin (7).
    Additional Material: 2 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    Metabolic engineering of cultured tobacco cells (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 329-332 
    Details
    ISSN: 0006-3592
    Keywords: tobacco cultured cells ; heat-shock promoter of Arabidopsis thaliana ; strong promoter from tobacco cell ; β-glucuronidase ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Construction of a gene expression system in tobacco cultured cells (BY2) was studied. A 925 bp promoter fragment of a heat-shock protein gene (HSP18.2) of Arabidopsis thaliana showed clear heat-shock response of expression of the β-glucuronidase (GUS) reporter gene in BY2 cells. Similar results were observed in a 500 mL flask and 3-L jar fermentor.Isolation of strong promoters in BY2 cells was tried. cDNA clones, in which the mRNA level is high in log-phase cells and the copy number in the genome is low, were isolated. These clones showed high homology with F1-ATPase (mitochondria type), elongation factor 1-α, and a gene with an unknown function of A. thaliana (clone 27), respectively. A 5′-flanking region of clone 27 showed 6.2 times the promoter activity of the CaMV35S promoter in BY2 cells.Three cDNA clones, which are expressed in the stationary growth phase of BY2 cells, were isolated by a differential screening. These clones showed high sequence homologies to alcohol dehydrogenase, pectin esterase, and extensin. Promoters of these genes will be useful in gene expression in high cell-density culture. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:329-332, 1998.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    NMR study of adsorbed water on acrylic acid-grafted and methacrylic acid-grafted cellulose (1972)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 16 (1972), S. 2197-2205 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: The water-adsorbing properties of cellulose samples which had been graft copolymerized with hydrophilic vinyl monomers using ceric salt as an initiator were investigated by means of the width of the high-resolution NMR absorption spectrum, water retention, and moisture regain of the samples. In the change of the width at half-value of the spectrum with per cent grafting, a maximum value followed by a minimum value was observed on samples both grafted with acrylic acid and methacrylic acid. Thus, the effect of grafting on the water-adsorbing properties appeared not to be simple. Though no special change in the water retention by grafting was observed, the per cent grafting dependence on the moisture regain in high relative humidity showed a peculiar and definite relation. The per cent grafting giving maximum moisture regain was about 8% and 18% for acrylic acid and methacrylic acid, respectively, and this agreed very well with the minimum widths at half-value. The change in the glass transition temperature of the sample - water system caused by grafting is also described.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1972.070160904
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