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  • 1
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Conditions for high-cell-density fermentations of Saccharomyces cerevisiae strains producing recombinant-DNA-derived proteins were established. Strains producing human immune interferon (IFN-γ) from the constitutive PGK promoter failed to grow to high cell densities and exhibited low plasmid stability. Regulated expression of IFN-γ was obtained in similar strains by employing a hybrid yeast GPD promoter that was subject to carbon source regulation due to the presence of regulatory DNA sequences from the yeast GAL 1,10 intergenic region. IFN-γ expression programmed by this vector was low during growth on glucose and was induced by galactose. Previously defined fermentation conditions employing glucose as a carbon source were applied to this strain, resulting in high ceil densities with higher plasmid stability. Various methods of galactose induction of IFN-γ expression in high-cell-density fermentations were investigated. Optimal conditions resulted in a 2000-fold induction and production of 2 g IFN-γ/L fermentation culture.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    International Journal of Quantum Chemistry 64 (1997), S. 523-552 
    ISSN: 0020-7608
    Keywords: Chemistry ; Theoretical, Physical and Computational Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The proper treatment of many-body effects for fermions has long been a goal of theorists working in atomic and molecular physics. The computational demands of such a treatment, however, when coupled to the added difficulties imposed by the presence of external electromagnetic sources, have resulted in few studies of many-body effects in strong magnetic fields, i.e., in the field regime where perturbation theory is no longer applicable. In this article, we review the fundamental aspects of the problem and describe a variety of theoretical approaches for small atoms and molecules in strong fields, beginning with mean-field theory (Hartree-Fock) and progressing through variational and exact stochastic methods.   © 1997 John Wiley & Sons, Inc. Int J Quant Chem 64: 523-552, 1997
    Additional Material: 17 Ill.
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  • 3
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    punctum books
    Publication Date: 2024-03-26
    Description: "From a stage erected in front of the US Capitol, on April 25, 1993, Michael Callen surveyed the throng: an estimated one million people stretched across the National Mall in the largest public demonstration of queer political solidarity in history. “What a sight,” he told the crowd, his earnest Midwestern twang reverberating through loudspeakers. “You’re a sight for sore eyes. Being gay is the greatest gift I have ever been given, and I don’t care who knows about it.” He then launched into a gorgeous rendition of “Love Don’t Need a Reason,” the AIDS anthem he composed with Marsha Malamet and the late Peter Allen. As Callen finished singing, people stood cheering and flashing the familiar American Sign Language symbol for “I Love You.” For they knew the song’s sentiment rang true for Callen, who had recently announced his retirement from music and activism after a living for more than a decade with what was then called “full-blown AIDS.” After the March on Washington, Callen returned to his recently adopted West Coast home, Los Angeles. In the ensuing months, his health rapidly declined, and on 27 December 1993, Callen died of AIDS-related pulmonary Kaposi’s sarcoma. Love Don’t Need a Reason focuses on Callen’s most important and lasting legacy: his music. A witness to the overlooked last years of Gay Liberation and a major figure in the early years of the AIDS crisis, Michael Callen chronicled these experiences in song. A community organizer, activist, author, and architect of the AIDS self-empowerment movement, he literally changed the way we have sex in an epidemic when he co-authored one of the first safe-sex guides in 1983. A gifted singer, songwriter, and performer, he also made gay music for gay people and used music to educate and empower people with AIDS. Listening again to his music allows us to hear the shifting dynamics of American families, changing notions of masculinity, gay migration to urban areas, the sexual politics of Gay Liberation, and HIV/AIDS activism. Using extensive archival materials and newly-conducted oral history interviews with Callen’s friends, family, and fellow musicians, Matthew J. Jones reintroduces Callen to the history of LGBTQIA+ music and places Callen’s music at the center of his important activist work."
    Keywords: Michael Callen ; HIV/AIDS ; popular music ; LGBTQ ; history ; LGBTQ activism ; thema EDItEUR::D Biography, Literature and Literary studies::DN Biography and non-fiction prose::DNB Biography: general::DNBF Biography: arts and entertainment ; thema EDItEUR::J Society and Social Sciences::JB Society and culture: general::JBS Social groups, communities and identities::JBSJ LGBTQ+ Studies / topics ; thema EDItEUR::M Medicine and Nursing::MJ Clinical and internal medicine::MJC Diseases and disorders::MJCJ Infectious and contagious diseases::MJCJ2 Medicine: HIV/AIDS, retroviral diseases
    Language: English
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    Format: image/jpeg
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