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  • Chemistry  (2)
  • Inorganic Chemistry  (1)
  • 1
    Electronic Resource
    Electronic Resource
    On-line monitoring of monoclonal antibodies in animal cell culture using a grating coupler (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 42 (1993), S. 1287-1292 
    Details
    ISSN: 0006-3592
    Keywords: integrated optics ; grating coupler ; FIA ; on-line monitoring ; animal cell culture ; monoclonal antibody ; immunochemical sensor ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A grating coupler was used for the on-line determination of monoclonal antibodies produced in perfused animal cell bioreactor. The device was connected with the culture vessel via a flow-injection analysis (FIA) system, which was controlled automatically. Specific antimouse lgG antibodies were immobilized on the surface of the sensor-chip. After injection of the sample, the binding of mouse lgG was observed in real time. The regeneration of the binding sites of the immobilized antibodies using an acidic solution allowed the on-line detection of produced monoclonal antibodies in the range of 10 to 150 μg/mL. In contrast to other techniques coupled to bioprocesses, the developed method represents a regenerable direct immunosensor. Results were compared with standard ELISA techniques (off-line) and a competitive immunochemical assay using the grating coupler (off-line). © 1993 John Wiley & Sons, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260421105
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  • 2
    Electronic Resource
    Electronic Resource
    Darstellung und Kristalldaten von LiBa2EuO4 (1967)
    Weinheim : Wiley-Blackwell
    Zeitschrift für anorganische Chemie 354 (1967), S. 27-29 
    Details
    ISSN: 0044-2313
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: The new quaternary compound LiBa2EuO4 may be prepared from stoichiometric amounts of LiEuO2 and BaO by heating at 700°C under an inert atmosphere. According to Debye-Scherrer photographs. LiBa2EuO4 and LiEu3O4 are isostructural (space group Pbnm). The orthorhombic unit cell with a = 11.883, b = 12.130 and c = 3.601 Å contains 4 formula units LiBa2EuO4 (calculated density 6.369 g/cm3).
    Notes: Die neue quaternäre Verbindung LiBa2EuO4 kann aus den stöchiometrisch erforderlichen Mengen LiEuO2 und BaO durch Erhitzen bei 700°C unter Schutzgas erhalten werden. Wie aus DEBYE-SCHERRER-Aufnahmen hervorgeht, ist LiBa2EuO4 mit LiEu3O4 isotyp (Raumgruppe Pbnm). Die rhombische Elementarzelle mit a = 11,883, b = 12,130 und c = 3,601 Å enthält 4 Formeleinheiten LiBa2EuO4 (berechnete Dichte 6,369 g/cm3).
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/zaac.19673540107
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