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Transcriptional regulation of hydroxypyruvate reductase gene expression by cytokinin in etiolated pumpkin cotyledons (1996)

Andersen, Brian R. ; Jin, Ge ; Chen, Rui ; [et al.]

Springer

Planta 198 (1996), S. 1-5

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ISSN: 1432-2048

Keywords: Cucurbita ; Cytokinin ; Gene expression ; Hydroxypyruvate reductase ; Transcription

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract To understand the mechanisms by which the expression of a specific gene is modulated by cytokinin, the regulation of hydroxypyruvate reductase (HPR) transcript levels by N6-benzyladenine (BA) in etiolated pumpkin (Cucurbita pepo L. cv. Halloween) cotyledons was investigated. A pumpkin HPR cDNA was generated by reverse transcriptase-polymerase chain reaction and its nucleotide sequence was determined. An antisense HPR RNA was prepared for RNase protection analysis of HPR-mRNA expression patterns in the cotyledons of dark-grown pumpkin seedlings. Treatment of the cotyledons with BA was shown to modulate HPR mRNA levels in a dose- and time-dependent manner. Similarly, nuclear run-on studies showed that the rate of transcription was also enhanced by BA treatment of the cotyledons. These results suggest that the enhancement of HPR mRNA by cytokinin is, at least in part, at the level of transcription.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00197579

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Studies on polyurethane protective surface material resistance to cavitation erosionThe following three Institutions take part in our research work: Sichuan Centre Institute of Electric Power, Sichun Institute of Water Conservancy Science, and Dong Fang Motorltenator Plant. (1982)

Xiong, Xiao Han ; Min, Gao Xue ; Tao, Zhou Qi ; [et al.]

Stamford, Conn. [u.a.] : Wiley-Blackwell

Polymer Engineering and Science 22 (1982), S. 752-759

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ISSN: 0032-3888

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: The polyurethane protective surface material, which were made from four different types of polyester and polyether polyol, have been studied with respect to their ultimate properties. The PTMG (polyoxytetramethylene glycol)- and PPG (polyoxypropylene giyco)-based polyurethane protective surface materials exhibited the best results. Use of JIPNs (joint interpenetrating polymer networks), prepared from combinations of polyurethnes with an epoxy adduct with coupling agents and talc, could clearly improve the adhesive strength resistance to water in negative pressure cavitation erosion (by determination with a rotating dish apparatus) and the adhesive strength resistance toward boiling water. The polyurethane protective surface material, which was composed of three separated layers, showed better resistance to cavitation erosion than an epoxy system, poly(methyl methacrylate), aluminum, steel, and different alloys in tests using the venturi tube and the rotating dish apparatus for simulated cavitation erosion, as well as in some power stations in actual field trials.

Additional Material: 11 Ill.