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Physical mapping of the Esterase-6 locus of Drosophila melanogaster (1991)

Procunier, W. S. ; Smith, J. J. ; Richmond, R. C.

Springer

Genetica 84 (1991), S. 203-208

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ISSN: 1573-6857

Keywords: Drosophila melanogaster ; Esterase-6 ; Est-6/Est-P duplication ; in situ hybridization ; polytene chromosomes ; gene mapping

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The Esterase-6 gene locus of Drosophila melanogaster although well-characterized, has not been definitly mapped by in situ hybridization. In this paper, a high resolution in situ hybridization protocol using an avidin/biotinylated-horseradish peroxidase/diaminobenzidine system was adopted to refine the physical map position of the Esterase-6 locus. Clarity of signal, detail of banding pattern and absence of background allowed the assignment of a 1.8 kb cDNA encoding Esterase-6 to three bands within subsections 69 A1–A3 on the left arm of polytene chromosome 3. These data refine earlier deletion mapping and low resolution in situ hybridization results, which assigned Esterase-6 to 69 A1–A5. The potential use of this high resolution in situ hybridization technique in the analysis of the physical organization of the Esterase-6 gene duplication and surrounding region is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00127248

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A procedure for determining the concentration of Hydroxyl Groups on Silica Surfaces (1972)

Madeley, J. D. ; Richmond, R. C.

Weinheim : Wiley-Blackwell

Zeitschrift für anorganische Chemie 389 (1972), S. 92-96

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ISSN: 0044-2313

Keywords: Chemistry ; Inorganic Chemistry

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Description / Table of Contents: Durch Bestimmung der Konzentration von D2O in flüssigen Mischungen von D2O/H2O war es möglich, ein Verfahren zu entwickeln, um die Konzentration von Hydroxylgruppen auf der Oberfläche von Silica-Gel zu bestimmen. Die erhaltenen Werte von 4,4 ± 0,2 OH-Gruppen pro 100 Å2 Oberfläche stimmen mit den Ergebnissen anderer Autoren gut überein.

Notes: By determining the concentration of D2O in D2O/H2O liquid mixtures, it has been possible to develop a method to obtain the concentration of hydroxyl groups on the surface of silica gel. The results of 4.4 ± 0.2 -OH groups per 100 Å2 of surface are in good agreement with the findings of other workers.

Additional Material: 5 Ill.