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The effect of deuterium oxide on protein turnover in Lemna minor (1979)

Cooke, R. J. ; Grego, S. ; Oliver, J. ; [et al.]

Springer

Planta 146 (1979), S. 229-236

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ISSN: 1432-2048

Keywords: Density labelling ; Deuterium oxide ; Lemna ; Protein turnover

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Lemna minor fronds transferred to a sterile culture medium containing 50% (v/v) deuterium oxide (2H2O) rapidly undergo a loss of soluble protein with a corresponding increase in free amino acids. The loss of protein is due to two factors: (i) the inhibition of protein synthesis for 4 h followed by a slower rate of synthesis than normal, (ii) a rapid 9–10 fold increase in protein degradation. In plants grown for longer periods (3–6 days) in 50% 2H2O medium, protein synthesis is inhibited by 20% and the rate constant of degradation is 2–3 times that measured in fronds growing in normal (H2O containing) complete medium. The initial loss of protein is not due to the breakdown of any specific protein fraction. Investigation of several enzymes indicates that all proteins are catabolised in response to 2H2O treatment. The implications of these results with regard to the interpretation of density-labelling experiments are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00388237

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Fractionation of polyethylenePresented in part at the 130th National American Chemical Society Meeting, Atlantic City, September 19, 1956. (1958)

Francis, P. S. ; Cooke, R. C. ; Elliott, J. H.

Hoboken, NJ : Wiley-Blackwell

Journal of Polymer Science 31 (1958), S. 453-466

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ISSN: 0022-3832

Keywords: Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Physics

Notes: The relatively rapid Desreux technique for polymer fractionation has been modified and applied to both linear and highly branched polyethylenes. The polymer is precipitated from p-xylene solution onto a sand column by cooling from 127° to room temperature. After removal of solvent, the column temperature is again raised to 127° and fractions are eluted, using p-xylene-ethylene glycol monoethyl ether mixtures of increasing solvent power. Approximately 15 fractions are obtained and polymer recovery is essentially quantitative. The reproducibility of the integral distribution curves is within ±2% for a cumulative weight-% of 85 or higher. In certain cases difficulty has been encountered in resolving the less soluble portion of the sample. The selective nature of the system is indicated by the fact that the reduced specific viscosities of the highest and lowest molecular weight fractions are generally in the ratio of 50 or higher.

Additional Material: 8 Ill.